經改造之油體鈣蛋白(caleosins)及其用於半抗原之抗體製造Engineered caleosins and its use in antibody production of hapten

專利類型

發明

專利國別 (專利申請國家)

中華民國

專利申請案號

100121116

專利證號

I 488865

專利獲證名稱

經改造之油體鈣蛋白(caleosins)及其用於半抗原之抗體製造Engineered caleosins and its use in antibody production of hapten

專利所屬機關 (申請機關)

國立中興大學

獲證日期

2015/06/21

技術說明

本發明係關於一種經改造之重組油體鈣蛋白，其特徵在於具有至少8個額外加入的賴胺酸(Lys)殘基。本發明進一步關於一種半抗原小分子之抗體製造方法，包括將該半抗原與本發明之經改造的重組油體鈣蛋白結合，將已併入該半抗原之重組油體鈣蛋白與三酸甘油酯及磷脂質建構成人造油體，然後將製得之人造油體免疫動物以製造對抗該半抗原之抗體。本發明首先試圖對油體鈣蛋白進行改良，使重組之油體鈣蛋白與半抗原小分子結合，並藉此重組油體鈣蛋白將該半抗原展現於人造油體的表面，而利於在受免疫的動物體內產生專一性對抗半抗原，而不會辨識載體蛋白之抗體。 A series of recombinant caleosins, mutated with 3, 5, 8, 11, 13, 15, and 17 extra Lys residues and over-expressed in Escherichia coli, were used as carrier proteins to render biotin as a hapten on the surface of artificial oil bodies for antibody production. Biotinylation levels of the recombinant caleosins were step-wisely elevated as the number of extra Lys residues increased. Polyclonal antibodies against biotin were successfully generated in rats injected with artificial oil bodies constituted with each of the biotinylated caleosins. Moreover, those generated via the biotinylated caleosins with 8 or more extra Lys residues no longer recognized caleosin. It appears that engineered Lys-rich caleosins are suitable carrier proteins for the production of antibodies against small molecules.

備註

本部(收文號1090023163)同意該校109年4月20日興產字第1094300209號函申請終止維護專利(中興)

連絡單位 (專責單位/部門名稱)

技術授權中心

連絡電話

04-22851811

網址