Infertility control of genetically modified fishInfertility control of genetically modified fish

專利類型

發明

專利國別 (專利申請國家)

美國

專利申請案號

12/705,509

專利證號

US 8,383,880 B2

專利獲證名稱

Infertility control of genetically modified fishInfertility control of genetically modified fish

專利所屬機關 (申請機關)

中央研究院

獲證日期

2013/02/26

技術說明

為建立有效之基因轉殖魚類不孕方法，本發明係提供一種基因轉殖魚類之誘導性不孕平台技術，其係藉由將一可表現誘導性毒性蛋白之質體嵌合至 魚體內，使誘導性毒性蛋白專一表現於生殖細胞，藉由誘發毒性蛋白之功能， ，破壞該基因轉殖魚之生殖細胞，達到基因轉殖魚類百分之百不孕之目標，進而避免基因轉殖魚類流放野外所產生之生態衝擊問題。 為達前述目的，本發明提供一種基因轉殖魚類之誘導性不孕方法，其步驟包含：(1)構築一表現質體，該表現質體具有專一性於生殖細胞驅動基因表現之特性，係至少包括一可專一性於生殖細胞表現之啟動子，及一受生殖細胞專一性啟動子調控之基因序列，該基因序列可編碼出一蛋白質，該蛋白質可經由誘導方式產生細胞毒性(cytotoxin)造成生殖細胞死亡；(2)將表現質體嵌入一魚類染色體以產生一基因轉殖魚；以及(3)於該基因轉殖魚培育期間，以步驟(1)的誘導方式處理基因轉殖魚，使得基因轉殖魚體內於生殖細胞表現之步驟(1)的蛋白質產生細胞毒素而使生殖細胞死亡，以使該基因轉殖魚不孕。 The invention relates to a GM fish whose genome comprises: (a) a fish gonad-specific promoter; and (b) a foreign transgene linked to the fish gonad-specific promoter, in which the foreign transgene comprises a suicide gene selected from the group consisting of a reductase and a photosensitizer, and further wherein the fish gonad-specific promoter is selected from the group consisting of a fish ovary-specific promoter and a fish testis-specific promoter; and wherein a female of the fish that is linked to the ovary-specific promoter and a male of the fish that is linked to the testis-specific promoter; and further wherein infertility of the GM fish is induced and/or the probability of infertility of the GM fish is increased as compared to that of a wild-type counterpart if the GM fish expressing the reductase is treated with an effective amount of a reductase-activated cytotoxic prodrug, or if the GM fish expressing the photosensitizer is treated with light irradiation.

備註

本部(收文號1100010775)同意該院110年2月22日智財字第1100501424號函申請終止維護專利(中央研究院)

連絡單位 (專責單位/部門名稱)

智財技轉處

連絡電話

02-2787-2508

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