反向聚焦顯微成像結構及方法

專利類型

發明

專利國別 (專利申請國家)

中華民國

專利申請案號

101129807

專利證號

I 456255

專利獲證名稱

反向聚焦顯微成像結構及方法

專利所屬機關 (申請機關)

國立中央大學

獲證日期

2014/10/11

技術說明

本發明利用全像共軛技術，可在生醫組織內產生一道反向聚焦之探測光。一道檢測光(Probing beam)經由分光鏡(Beam splitter, B.S.)將光導向物鏡(Objective lens)，再將探測光聚焦至生物組織內，並利用此物鏡接收由生物組織內部之散射光，被物鏡接收之散射光將被引入一光學相位共軛反射鏡(PCM)內，而此PCM將此道散射光循原軌跡還原，此架構只需要架設於生醫組織之同一側，可在生醫組織內產生一道反向聚焦的共軛探測光，對於較厚光學無法穿透之組織亦可使用。若在產生反向聚焦之探測光同時引入原探測光，此兩道光對打產生之效應可應用在光鉗、螢光顯微鏡、4 pi顯微鏡、光動力刀與受激發射損耗顯微鏡(Stimulated Emission Depletion, STED)等研究。這項研究是至今仍未被提出的。 This invention use holographic conjugation technique to produce an inverse focusing beam inside the bio-tissue. In this technique, a probing beam is reflected by beam splitter and routed to the objective lens, and is focused inside the bio tissue. The scattering light inside caused by tissue is collected by objective lens and is directed to a phase conjugation mirror (PCM). This PCM produces the phase conjugated wave of the scattering light, and thus produces inverse focusing beam inside the tissue. Since we can produce focusing beam in counter-direction by just put the microscope in on side of the bio-tissue, it can be used to detect thicker bio-tissue. If we introduce the forward focusing light except to the inverse focusing light, the technique can be applied to Tweezer, Fluorescent microscopy, Confocal microscopy, 4pi microscopy, photo dynamic therapy, and Stimulated Emission Depletion (STED). This invention has never been proposed previously.

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