CELL CHIP AND DYNAMIC DIALYSIS STAINING FOR CELLS

專利類型

發明

專利國別 (專利申請國家)

美國

專利申請案號

16/795,511

專利證號

US 11,547,999 B2

專利獲證名稱

CELL CHIP AND DYNAMIC DIALYSIS STAINING FOR CELLS

專利所屬機關 (申請機關)

國立清華大學

獲證日期

2023/01/10

技術說明

細胞晶片包括第一與第二基板及位於兩者之間的染劑擴散層。染劑擴散層的細胞自組裝區對應第一基板的第一孔洞且與其之間具有間距。細胞自組裝區包括第二孔洞且用以容置含細胞之樣本液。第二孔洞孔徑小於細胞之粒徑，細胞單層排列於細胞自組裝區，而樣本液之液體經由第二孔洞進入第二基板的微流道結構。當染劑經由第一孔洞進入細胞自組裝區時，染劑與微流道結構中的洗滌液之間的濃度差使得染劑由細胞自組裝區朝向微流道結構擴散，藉此細胞被染劑染色，且洗滌液將經由第二孔洞進出，以加速染劑之擴散，因而達成高效率細胞動態透析染色。 【英文】A cell chip including a first substrate, a second substrate and a dye diffusion layer disposed therebetween is provided. A cell-assembly region of the dye diffusion layer is disposed corresponding to a first hole of the first substrate and separated from the first substrate by a spacing. The cell-assembly region includes second holes and is configured to contain a sample solution containing cells. A size of the second hole is smaller than a size of the cell, and the cells are arranged onto the cell-assembly region in one cell layer manner and solution of the sample solution flows into a micro-channel structure of the second substrate. When a dye is injected to the cell-assembly region by passing through the first hole, the dye diffuses from the cell-assembly region to the micro-channel structure due to a concentration difference between the dye and a washing solution flowing in the micro-channel structure, and the cells are dyed. The washing solution enters into and leaves from the second holes to accelerate the diffusion of the dye, and thus a high efficiency dynamic dialysis staining for cells is achieved.

備註

本會(收文號1120009610)同意該校112年2月16日清智財字第1129001195號函申請專利讓與(清華大學)

連絡單位 (專責單位/部門名稱)

智財技轉組

連絡電話

03-5715131-62219

網址