菌株快篩方法以及該方法所使用的培養基

專利類型

發明

專利國別 (專利申請國家)

中華民國

專利申請案號

106102639

專利證號

I 604049

專利獲證名稱

菌株快篩方法以及該方法所使用的培養基

專利所屬機關 (申請機關)

義守大學

獲證日期

2017/11/01

技術說明

保麗龍(聚苯乙烯)使用是造成地球不可分解之汙染的環保重要議題，但人類卻一直不斷的生產製造此類聚合物，導致保麗龍只多不少，造成污染量逐漸擴大，一般處理又容易產生戴奧辛，而相關生物分解菌種很難找出分解菌。 本團隊致力於篩選出菌種以及研究菌株所釋放的酵素平台之研究，由麵包蟲篩得TM-1分解菌及大麥蟲篩得ZM-1菌株，本團隊所建立分解聚苯乙烯之平台，建立後成功分離出了兩種菌株，TM-1及ZM-1，兩種菌株在混濁度試驗中都有使聚苯乙烯混濁度下降的趨勢。 本專利所建立的平台可於短時間內篩選出保麗龍分解菌種，可助於未來保麗龍污染的減少及再利用。 Under the observation that Tenebrio molitor and Zophobas morio could eat polystyrene (PS), we setup the platform to screen the gut microbes of these two worms. To take advantage of that Tenebrio molitor and Zophobas morio can eat and digest polystyrene as its diet, we analyzed these special microbes with PS plate and PS turbidity system with time courses. There were two strains TM1 and ZM1 which isolated from Tenebrio molitor and Zophobas morio, and were identified by 16S rDNA sequencing. The results showed that TM1 and ZM1 were cocci-like and short rod shape Gram-negative bacteria under microscope. The PS plate and turbidity assay showed that TM1 and ZM1 could utilize polystyrene as their carbon sources. The further study of PS degraded enzyme and cloning warrants our attention that this platform will be an excellent tools to explore and solve this problem.

備註

連絡單位 (專責單位/部門名稱)

產學智財營運總中心產學合作與專利技轉中心

連絡電話

07-6577711ext2683

網址