應用橋石短芽孢桿菌表現系統生產醣基化重組三葉因子胜肽之方法、醣基化重組三葉因子胜肽及其用途METHOD FOR PRODUCTION OF GLYCOSIDIC RECOMBINANT TREFOIL FACTOR 1 BY BREVIBACILLUS CHOSHINENSIS EXPRESSION SYSTEM, GLYCOSIDIC RECOMBINANT TREFOIL FACTOR 1 AND USES THEREOF

專利類型

發明

專利國別 (專利申請國家)

中華民國

專利申請案號

102145147

專利證號

I 472616

專利獲證名稱

應用橋石短芽孢桿菌表現系統生產醣基化重組三葉因子胜肽之方法、醣基化重組三葉因子胜肽及其用途METHOD FOR PRODUCTION OF GLYCOSIDIC RECOMBINANT TREFOIL FACTOR 1 BY BREVIBACILLUS CHOSHINENSIS EXPRESSION SYSTEM, GLYCOSIDIC RECOMBINANT TREFOIL FACTOR 1 AND USES THEREOF

專利所屬機關 (申請機關)

國立中興大學

獲證日期

2015/02/11

技術說明

三葉因子胜肽(Trefoil factors, TFFs) 促進黏膜損傷之修復。一般由胃及腸道之表皮細胞所分泌。三葉因子胜肽經由雙硫鍵構成之三葉構型及雙體結構，影響其傷口癒合能力。本發明以短短芽孢桿菌分泌生產重組三葉因子胜肽，可達高量分泌生產單體，雙體，三體及四聚體之三葉因子胜肽。一新穎性發現為，經由質體構築過程所創造之N端絲胺酸被醣基化。此醣基化重組三葉因子胜肽在模擬胃酸條件下，具有更佳傷口癒合能力。本發明提供一生產及純化三葉因子胜肽之方法，所生產之三葉因子胜肽可預防黏膜損傷及加速腸胃道傷口癒合進行。 Trefoil factors (TFFs) mediate mucosal repair. Trefoil factor 1 (TFF1) is a highlyconserved trefoil factor family protein that is secreted by the epithelial cells of thestomach and the colon mucous membranes. TFF1 forms homodimers via a disulfidelinkage that affects protein activity in wound healing assays. The recombinant human TFF1 (rTFF1) was expressed extracellularly by Brevibacillus choshinensis. The rTFF1 was secreted at high levels and formed monomers, dimers, trimers and tetramers. A glycosyl modification occurred on the N-terminal Ser residue of rTFF1 which was created by the constructing process of the secretion vector. The rTFF1 proteins of B. choshinensis were purified by Ni-NTA chromatography. The rTFF1 had better wound healing capacity when pretreated with pH 2.4 buffer that minic the stomach condition. This invention provides a powerful method of rTFF1 production that may protect against gastric damage and promote wounding healing.

備註

本會(收文號1120002852)同意該校112年1月9日興產字第1124300027號函申請終止維護專利(國立中興大學)

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