用於檢測多生理參數之細胞活性評估晶片A CELL-ACTIVITY ESTIMATION CHIP USED FOR DETECTING MULTI-PHYSIOLOGICAL PARAMETERS

專利類型

發明

專利國別 (專利申請國家)

中華民國

專利申請案號

098141983

專利證號

I 377345

專利獲證名稱

用於檢測多生理參數之細胞活性評估晶片A CELL-ACTIVITY ESTIMATION CHIP USED FOR DETECTING MULTI-PHYSIOLOGICAL PARAMETERS

專利所屬機關 (申請機關)

國立中興大學

獲證日期

2012/11/21

技術說明

本發明係為一種用於檢測多生理參數之細胞活性評估晶片，包括一感測晶片及一微流道墊高層，該感測晶片包括一輔助電極、一參考電極、二溶氧電極與二pH電極，於該參考電極與兩pH電極表面形成一氧化銥層；該微流道墊高層包括一墊高膜本體、一微流道層及一細胞培養槽，該墊高膜本體位於該感測晶片上，該微流道層結合於該墊高膜本體上並形成一微流道，該細胞培養槽貫穿於該微流道層及該墊高膜本體，使兩pH電極、兩溶氧電極與輔助電極自該細胞培養槽露出。如此，本發明能同時檢測細胞酸化率與呼吸活性，以評估細胞活性的變化。 This invention is a cell-activity estimation chip used for detection of multi-physiological parameters, which includes a sensing chip and a microchannel-levitated slab. The sensing chip contains a counter electrode, a reference electrode, two dissolved oxygen electrodes and two pH electrodes. Furthermore, an iridium oxide film is formed on the surface of the reference electrode and both the pH electrodes. The microchannel-levitated slab includes a spacer film, a microchannel layer and a cell culture chamber. The spacer film is placed on the sensing chip and combined with the microchannel layer to form a microchannel. The cell culture chamber penetrates the microchannel layer and the spacer film, and two pH electrodes, two dissolved electrodes and a counter electrode are exposed in the cell culture chamber. Therefore, the invention can simultaneously detect the cellular acidification rate and the respiratory activity to estimate the change in the cellular activity.

備註

本部(文號1090000062)同意該校108年12月31日興產字第1084300814號函申請終止維護專利(中興)

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