Virtual Spatial Overlap Modulation Microscopy For Resolution Improvement

專利類型

發明

專利國別 (專利申請國家)

美國

專利申請案號

14/474,167

專利證號

US 9,384,537 B2

專利獲證名稱

Virtual Spatial Overlap Modulation Microscopy For Resolution Improvement

專利所屬機關 (申請機關)

國立臺灣大學

獲證日期

2016/07/05

技術說明

vSPOM 是比使用反摺積技術較容易實現，因為它不需要使用到雜訊模型及系統點擴散函數的評估。而vSPOM對於在體內觀測的二倍頻訊號(SHG)及三倍頻訊號(THG)顯微術是特別有用，產生的影像不是藉由點擴散函數與物體的摺積，而是由在聚焦體積下非線性干涉交會作用的積分所產生。另外，我們也積極致力於影像分析軟體的開發。在計畫上半年我們完成了vSPOM的影像分析程式，並且將此成果發表在國際期刊上（W.-C. Kuo et al., Virtual spatial overlap modulation microscopy for resolution improvement. Opt. Exp. 21, 30007-18, 2013）。利用此分析軟體，我們可以大幅提高本產品之影像解析度。為了使本產品的人機介面可以更優化、更貼近使用者需求，我們也尋求臺大智活（iNSIGHT）的協助，請他們幫我們評估如何以使用者經驗為核心來開發此產品之人機介面。 As a post-processing technique, vSPOM is much easier to implement than deconvolution because it does not involve the estimation of noise model and system point spread function (PSF). In particular, vSPOM is especially useful for the applications of in vivo SHG (Second-Harmonic Generation) and THG (Third Harmonic Generation) microscopy, which forms image not by the convolution of PSF and object, but by the integral of nonlinear coherent interaction upon the focal volume. Multiple basic limits exist regarding its application on resolution improvement, including the limits on spatial frequency and the tradeoff between signal-to-noise ratio (SNR) and resolution. The highest spatial frequency attainable is limited by the distance between adjacent pixels, a parameter determined by hardware data acquisition procedure. On the other hand, small derivative mask gives us high spatial resolution but it tends to enhance noise and deteriorate SNR.

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