嗜鹽甲烷古菌Ｍｅｔｈａｎｏｈａｌｏｐｈｉｌｕｓ　ｐｏｒｔｕｃａｌ ｅｎｓｉｓ　ＦＤＦ１Ｔ之Ｓ－腺苷高半胱胺酸水解酵素基因及其於生產 Ｓ－腺苷高半胱胺酸之應用

專利類型

發明

專利國別 (專利申請國家)

中華民國

專利申請案號

101120990

專利證號

I 607084

專利獲證名稱

嗜鹽甲烷古菌Ｍｅｔｈａｎｏｈａｌｏｐｈｉｌｕｓ　ｐｏｒｔｕｃａｌ ｅｎｓｉｓ　ＦＤＦ１Ｔ之Ｓ－腺苷高半胱胺酸水解酵素基因及其於生產 Ｓ－腺苷高半胱胺酸之應用

專利所屬機關 (申請機關)

國立中興大學

獲證日期

2017/12/01

技術說明

本發明係應用高鹽甲烷太古生物Methanohalophilus portucalensis FDF1的兩套腺苷高半胱胺酸水解酵素(MpSAHH1& MpSAHH2)基因，利用重組基因技術將轉殖入大腸桿菌中大量表現以作為生產藥用腺苷高半胱胺酸(SAH)做為癲癇與睡眠調控用藥。此外，MpSAHH1氨基酸序列親緣與保守性和細菌型SAHH較相似具insertion sequences， MpSAHH2氨基酸序列之親緣與保守性與人類SAHH相似不具insertion sequences，此結構特色可用於設計抗細菌的腺苷高半胱胺酸水解酵素抑制劑。 The present invention relates to the isolated nucleic acid molecules encoding two-type of S-adenosylhomocysteine hydrolases (SAHH) from halophilic archaea-Methanohalophilus portucalensis FDF1. The heterologous overexpression of MpSAHH1 or MpSAHH2 could apply for S-adenosylhomocysteine (SAH), an effective sedative and a good sleep modulator), synthesis in vivo and in vitro. Additionally, amino acid sequence conservation and phylogenetic analysis indicated MpSAHH1 as bacterial SAHH which possess insertion sequences, whereas MpSAHH2 is related to human SAHH which did not have insertion sequences. This insertion sequence provide critical hydrophobic amino acids to pass substrate homocysteine to reach catalytic domain and this important information can be apply for SAHH inhibitor to kill the infected pathogen.

備註

本部(收文號1110019123)同意該校111年4月1日興產字第1114300232號函申請終止維護專利(國立中興大學)

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