發展成豬的重組胚囊、豬胎兒及仔豬之培育方法

專利類型

發明

專利國別 (專利申請國家)

中華民國

專利申請案號

102135530

專利證號

I 481716

專利獲證名稱

發展成豬的重組胚囊、豬胎兒及仔豬之培育方法

專利所屬機關 (申請機關)

中國醫藥大學

獲證日期

2015/04/21

技術說明

本案為利用取自屠宰場豬卵巢所分離之卵母細胞為受核卵質來源，經分切去核後可植入特定之供核體細胞，以產製複製動物(豬)或轉基因複製動物的技術平台。本技術的特點在於以最低的成本、人力與設備(設備成本需求可降低約5倍不等)，達到高效率(提高約0.5-2,0倍)產製複製胚與複製動物的方法。較之傳統複製方法，本技術可節省5-7倍的設備費與1-2倍之勞力，而維持相同或獲得更高的產製效率。本技術未來可取代傳統之體細胞核轉置(somatic cell nuclear transfer)技術在農業(動物)生產科技、保存頻臨絕種物種與生物醫學方面的應用。 The invention is to improve the quality of cloned porcine embryos by oocyte bisection and multiple embryo aggregations. Embryos derived from aggregation of three cloned embryos (3X) had a better blastocyst rate than cloned control (1X) embryos (73.6% vs. 35.1%, respectively; P < 0.05). The ratios of inner cell mass to total cell number were comparable in the 2X and 3X groups (25.1% vs. 26.1%, respectively) and were significantly better than that in the control group (15.3%). Expression of Oct4 and Cdx2 was higher, whereas that of Bax was lower (P < 0.05), in the 3X compared with non-aggregate group. Seven piglets were born to two surrogate mothers after embryo transfer of 3X aggregate blastocysts. In conclusion, aggregated embryos had greater total cell numbers and better pluripotency gene expression, with reduced expression of the pro-apoptosis gene Bax. Collectively, these improvements may be associated with development of cloned embryos to term.

備註

連絡單位 (專責單位/部門名稱)

產學合作處生物科技發展育成中心

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(04)2205-3366轉1581

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