針對低分子量及高極性之四級胺化合物之水性溶劑分散液液微萃取法

專利類型

發明

專利國別 (專利申請國家)

中華民國

專利申請案號

105127903

專利證號

I 583659

專利獲證名稱

針對低分子量及高極性之四級胺化合物之水性溶劑分散液液微萃取法

專利所屬機關 (申請機關)

高雄醫學大學

獲證日期

2017/05/21

技術說明

生物體內有許多具有生命意義的小分子化合物，但其水溶性極高且偵測靈敏度不佳，如肉鹼及其相關化合物具有診斷疾病、作為治療藥物、作為食品添加、作為化妝品活性成分等實質功能，但其結構不易使用螢光偵測器偵測且其水溶性極高，不容易從水樣品中萃取出來。因此我們利用4-bromomethyl biphenyl這個試劑與肉鹼及其相關化合物進行化學衍生反應，提高螢光偵測器的偵測能力，並利用水性溶劑分散液液微萃取法(aqueous solvent-based dispersive liquid-liquid microextraction)提高水溶性小分子的萃取能力。 此方法中探討了影響衍生及萃取效率之參數，定量線性範圍為10-2000 nM，偵測極限於3 nM。同日內與異日間分析精密度(相對標準偏差)與精確度(相對誤差)小於5.5 %。我們成功地將此萃取及分析法應用於藥品、化粧品、食品、人體血漿中分析肉鹼、丁基甜菜鹼及乙醯肉鹼等水溶性待測物。 L-carnitine and its relative compounds can be used in many field such as fatty acid disorder diagnosis, pharmaceuticals, food additives, and active ingredients in cosmetics. According to their physical and chemical properties, 4-bromomethyl biphenyl was utilized as a chemical derivatization reagent to improve the detection sensitivity. The aqueous solvent-based dispersive liquid-liquid microextraction was employed to enhance the extractability in various samples. Several parameters of these derivatization and extraction efficiency were explored. The linear range of these analytes was 10-2000 nM and the limitation of detection were about 3 nM. Intraday and interday analysis with precision (relative standard deviation) and accuracy (relative error) are both less than 5.5%. This method is successfully applied in pharmaceuticals, cosmetics, food and human plasma for butyrobetaine, L-carnitine and acetyl-L-carnitine analysis.

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