逆境誘導表現系統及其用途A STRESS RESPONSE SYSTEM ANE USE THEREOF

專利類型

發明

專利國別 (專利申請國家)

中華民國

專利申請案號

104121780

專利證號

I 567196

專利獲證名稱

逆境誘導表現系統及其用途A STRESS RESPONSE SYSTEM ANE USE THEREOF

專利所屬機關 (申請機關)

國立臺灣海洋大學

獲證日期

2017/01/21

技術說明

過去，本研究團隊以三角褐指藻 (Phaeodactylum tricornutum) 做為模式生物，成功發現三角褐指藻的鹼性磷解酶 (alkaline phosphatase，簡稱 PtAPase) 基因會受到缺磷逆境的誘導，而大量進行基因表現。由此原創的發現，我們進一步鑑定與選殖了 PtAPase 的啟動子 (promoter)，並以此開發出了可受磷酸鹽濃度誘導調控的矽藻表現載體 (Vector pPtAP)。 (1) 相較於其它的矽藻表現載體 pPha 與 pNR，我們開發的 pPtAP 載體所表現出的 EGFP 外源性基因重組蛋白能夠高出 13 倍或17 倍。 (2) 在基因轉殖矽藻細胞中重組蛋白質的數目高達總蛋白量的 50 % 以上。 Here, we identified a functional promoter of PtAPase of the alkaline phosphatase (pPtAPase), which is highly induced in Phaeodactylum tricornutum. By using it, we also developed a novel diatom expression vector (pPtAP), which can induce exogenous gene’s expression abundantly when cells are phosphorus starved. Compared with other diatom’s expression vectors, such as pPha and pNR, our pPtAP vector expressed 13- and 17-fold of EGFP exogenous recombinant proteins, respectively. Moreover, the abundance of recombinant proteins is as much as 50 % of total protein in the transgenic diatom. 　　Diatoms are considered as an ideal expression system for complex recombinant proteins, such as protein drugs, due to their low cost in production and highly post-translation modification ability. With our powerful pPtAP vector, we could make diatoms a better “Pharm”.

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