檢測生物胺的質譜分析方法

專利類型

發明

專利國別 (專利申請國家)

中華民國

專利申請案號

107137944

專利證號

I 696832

專利獲證名稱

檢測生物胺的質譜分析方法

專利所屬機關 (申請機關)

國立中山大學

獲證日期

2020/06/21

技術說明

本技術結合探針採樣法、熱脫附法(Thermal Desorption, TD)及電噴灑游離法(Electrospray Ionization, ESI)開發出可直接、快速分析樣品表面或內部生化分子的質譜分析技術，稱之為熱脫附電噴灑游離質譜法(Thermal Desorption Electrospray Ionization Mass Spectrometry, TD-ESI/MS)。分析時使用金屬細針輕戳樣品進行採樣，蒐集來自海鮮所產生的生物胺(例如腐胺、屍胺、腐胺)及胺基酸，再將探針直接插入預熱的高溫爐中，此時附著於探針上的分析物因高溫環境而進行熱脫附，產生中性氣相待測分子，最後藉由熱氣流將氣相待測分子傳送到電噴灑離子雲中進行後游離(post-ionization)，產生分析物離子，並使用質量分析器進行偵測。 本技術可成功地自海鮮檢測出生物胺、胺基酸，分析速度快、時間短，且不需要均質、衍生化等樣品前處理。此技術可應用於海鮮食品的新鮮度鑑定、探討檢體中生物胺隨時間變化的趨勢、判斷生物死亡時間(腐敗程度)。 This technique combined probing sampling, thermal desorption (TD), and electrospray ionization/mass spectrometry (ESI/MS) for direct and rapidly analysis of small biological compounds in sample or on sample surface, termed as thermal desorption electrospray ionization mass spectrometry (TD-ESI/MS). Biological chemical compounds in seafood such as biogenic amines and amino acids were sampled by using a fine metal probe, which were then introduced into a heated oven for evaporating analytes. After that, the thermally desorbed analytes were introduced into an ESI plume for post-ionization and the following MS detection. This approach showed features of direct and rapid analysis of biogenic amines and amino acids without tedious sample preparation such as homogenization and derivatization. So that, TD-ESI/MS could be applied to determine seafood freshness assessment, the trend of biogenic amines over time in samples, and forensic death time evaluation.

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