用於細胞呼吸活性評估並結合圖案化細胞培養之溶氧電極陣列晶片及其製法A DISSOLVED-OXYGEN ELECTRODE ARRAY WITH THE INTEGRATION OF CELL-PATTERNIZED CULTURE USED FOR ESTIMATION OF CELLULAR RESPIRATORY ACTIVITY AND ITS FABRICATION METHOD

專利類型

發明

專利國別 (專利申請國家)

中華民國

專利申請案號

098141985

專利證號

I 393882

專利獲證名稱

用於細胞呼吸活性評估並結合圖案化細胞培養之溶氧電極陣列晶片及其製法A DISSOLVED-OXYGEN ELECTRODE ARRAY WITH THE INTEGRATION OF CELL-PATTERNIZED CULTURE USED FOR ESTIMATION OF CELLULAR RESPIRATORY ACTIVITY AND ITS FABRICATION METHOD

專利所屬機關 (申請機關)

國立中興大學

獲證日期

2013/04/21

技術說明

本發明係為一種結合圖案化細胞培養之溶氧電極陣列晶片，包括一感測晶片及一微流道墊高層，該感測晶片包括一基材以及一設置於基材上的絕緣層，該基材上係設有一細胞貼附區、一輔助電極及複數個溶氧電極，於各溶氧電極與該輔助電極設有一工作窗口，該絕緣層覆蓋住該輔助電極及複數個溶氧電極上並穿設一細胞培養孔以露出該細胞貼附區，該細胞貼附區中心與該工作窗口的距離小於該細胞貼附區半徑的五倍，該微流道墊高層結合於該感測晶片上。本發明之溶氧電極陣列分佈在細胞圖案區之半球形溶氧消耗擴散半徑內，可量化分析細胞的呼吸速率。 The invention is a dissolved-oxygen (DO) electrode array chip integrated with cell-patternized culture, which consists of a sensing chip and a microchannel-levitated slab. The sensing chip includes a substrate containing a cell-cultured area, a counter electrode and multi-DO electrodes and an insulator layer placed on the substrate. Each DO electrode and the counter electrode have a working window. The insulator layer covers the counter electrode and the multi-DO electrodes, and a cell-culture hole, which is used as a cell-adhesion region, is placed on the insulator. The distance between the center of the cell-adhesion region and the working window is smaller than the five times radius of cell-cultured area. The microchannel-levitated layer is combined with the sensing chip. In the invention, the DO electrode array is distributed within the hemispherical diffusion radius of oxygen consumption of cell-cultured area, and it can quantify the respiratory rate of cells.

備註

本部(收文號1070026132)同意該校107年4月18日興產字第1074300247號函申請終止維護專利

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