將脂肪幹細胞培養成球狀體並使其神經分化之培養材料及方法Material and method for culturing adipose-derived stem cells into adipose-derived stem cells and trans-differentiating into neural lineage

專利類型

發明

專利國別 (專利申請國家)

中華民國

專利申請案號

101124286

專利證號

I 490337

專利獲證名稱

將脂肪幹細胞培養成球狀體並使其神經分化之培養材料及方法Material and method for culturing adipose-derived stem cells into adipose-derived stem cells and trans-differentiating into neural lineage

專利所屬機關 (申請機關)

國立成功大學

獲證日期

2015/07/01

技術說明

本發明係有關於一種球狀體脂肪幹細胞培養材料，係包括一基底；以及表面塗佈一幾丁聚醣(chitosan)薄膜，該幾丁聚醣薄膜係形成於該基底上，且係包含65-90%之去乙醯化度(degree of deacetylation)，並且該幾丁聚醣薄膜表面之最高突出及最低凹陷之一高度差係介於1-25 nm間。此外，本發明更提供一種以上述材料使動物或人體所分離之脂肪幹細胞進行體外培養，並誘導形成球狀體並轉分化成神經相關細胞之方法，且無須額外添加各種營養誘導因子。藉由將成體幹細胞之脂肪幹細胞培養成具有分化能力之細胞球狀體，且在誘導該球狀體神經轉分化情況下，能有效應用於多種神經損害相關之修復。 The present invention relates to a material for spheroid formation of adipose-derived stem cells, comprising a substrate; and a chitosan film, which is surface coating and formed on the substrate, wherein the chitosan film has 65-90% degree of deacetylation, and the height difference between the highest and lowest position of the chitosan film is 1-25 nm. In addition, the present invention further provides a method for inducing the spheroid trans-differentiation into the neural lineage by the present method.

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