一種篩檢拓樸異構酶抑制劑之裝置及其方法sensor chip for screening topoisomerase inhibitor and screening method thereof

專利類型

發明

專利國別 (專利申請國家)

美國

專利申請案號

12/924,118

專利證號

US 9,005,922 B2

專利獲證名稱

一種篩檢拓樸異構酶抑制劑之裝置及其方法sensor chip for screening topoisomerase inhibitor and screening method thereof

專利所屬機關 (申請機關)

臺北醫學大學

獲證日期

2015/04/14

技術說明

本發明係揭露一種篩檢抗癌藥物之裝置及其方法，本發明之裝置包括一生物晶片及一拓墣異構酶(topoisomerase)，拓墣異構酶係固定於該生物晶片上，且拓墣異構酶本身具有一催化活性。當一雙股去氧核醣核酸(DNA)與一抗癌藥物共同加入具有拓墣異構酶之生物晶片上時，藉由一表面電漿共振(SPR)分析抗癌藥物是否接合於該拓墣異構酶與雙股去氧核醣核酸。若是，抗癌藥物係具有抗拓墣異構酶之活性，進而阻斷雙股去氧核醣核酸之轉錄或複製。 The present invention discloses a device of screening an anti-cancer drug and a method thereof. The device of the present invention comprises a biochip and topoisomerase immobilized on the biochip, and the topoisomerase has a catalytic activity. When a double strand DNA and an anti-cancer drug are added onto the biochip comprising the topoisomerase, whether the anti-cancer drug connected to the topoisomerase and the double strand DNA or not can be detected by a surface plasmon resonance (SPR) anaylsis. If yes, the anti-cancer drug has an anti-topoisomerase activity, and blocks the transcription and replication of the double strand DNA.

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