麥角硫因應用於活化細胞之Nrf2訊息途徑之用途

專利類型

發明

專利國別 (專利申請國家)

中華民國

專利申請案號

103130810

專利證號

I 549696

專利獲證名稱

麥角硫因應用於活化細胞之Nrf2訊息途徑之用途

專利所屬機關 (申請機關)

中國醫藥大學

獲證日期

2016/09/21

技術說明

陽光紫外線A (UVA)可進入室內穿透到真皮層，是人體皮膚老化、皺紋生成、曬黑及皮膚癌的主因。皮膚細胞經UVA照射會引起細胞內活性氧(ROS)產生、DNA損傷和脂質過氧化等反應，最後造成細胞凋亡。本專利發現麥角硫因可保護人類皮膚角質細胞對抗UVA造成的ROS產生、DNA損傷、脂質過氧化和細胞凋亡等光損傷。本專利發現麥角硫因可促進Nrf2轉位入細胞核，增加抗氧化基因Nrf2轉錄，調節Nrf2下游抗氧化蛋白(HO-1和GCLC)活性，提高抗氧化能力，因而保護皮膚角質細胞降低 UVA的氧化傷害。以siNrf2抑制Nrf2活性，會使麥角硫因造成的抗氧化保護作用被抑制。總結，麥角硫因可作為化妝保養品或保健食品，保護人類皮膚細胞降低UVA引起的皮膚光老化。 UV radiation from the sun is a potent environmental risk factor in the pathogenesis of skin damage. Much of the skin damage caused by ultraviolet A (UVA) irradiation from the sun is associated with oxidative stress. The aim of this study was to investigate the protective role of ergothioneine (2-8 nM), a natural antioxidant, against UVA-induced oxidative stress and apoptosis in human keratinocyte (HaCaT) cells and to reveal the possible mechanisms underlying this protective efficacy. The antioxidant potential of ergothioneine was directly correlated with the increased expression of HO-1 and GCLC, which was followed by the downregulation of Keap1 and the augmented nuclear translocation and transcriptional activation of Nrf2 with or without UVA irradiation. Nrf2 knockdown diminished the protective effects of ergothioneine. Therefore, ergothioneine may be useful for the treatment of UVA-induced skin damage.

備註

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