NON-OBESE DIABETES MICE AND ITS APPLICATIONS

專利類型

發明

專利國別 (專利申請國家)

美國

專利申請案號

15/364,228

專利證號

US 10,634,666 B2

專利獲證名稱

NON-OBESE DIABETES MICE AND ITS APPLICATIONS

專利所屬機關 (申請機關)

臺北醫學大學

獲證日期

2020/04/28

技術說明

若能表現報告基因於非肥胖型糖尿病鼠(NOD mice)之胰島細胞，可協助研究者以非侵犯性造影方式即時追蹤糖尿病胰島凋亡過程，克服傳統方法必須犧牲大量NOD mice進行胰島切片觀察之缺點，對於自體免疫型糖尿病之研究相當重要。我們運用人類胰島素啟動子成功地建立胰島穩定表現抗聚乙二醇膜抗體報告基因(anti-PEG reporter)之NOD/pIns-αPEG mice。利用螢光顯微鏡與光學造影系統皆證實PEG-螢光探針可專一性結合並且顯影於NOD/pIns-αPEG mice之胰島區域，而非NOD mice對照組；證實NOD/pIns-αPEG mice可應用於非侵犯性造影追蹤自體免疫糖尿病之病程。表現anti-PEG reporter於NOD mice之胰島不會影響其胰島型態、大小、及單位面積內胰島數量；NOD/pIns-αPEG mice之自體免疫病程、胰島遭受免疫細胞浸潤情形、葡萄糖代謝效率、及自體抗體免疫反應，皆與對照組NOD mice無異；顯示anti-PEG reporter不會影響NOD/pIns-αPEG mice生理與病理特性。此NOD/pIns-αPEG mice可協助研究者以非侵犯性造影方式追蹤NOD mice罹患第一型糖尿病之病程，進一步為全世界藥廠與藥物研發單位提供一種糖尿病藥物篩選平台與療效評估工具。 Expressing a reporter gene in the pancreatic islet of non-obese diabetic mice (NOD mice) allows researchers real-time monitoring the processes of islet loss by noninvasive imaging systems. We have successfully developed the NOD/pIns-αPEG mice which stably express the anti-PEG reporter in their pancreatic islets by using an insulin promoter. The PEG-NIR797 fluorescent probe can specifically accumulate at the pancreatic islet region of NOD/pIns-αPEG mice but not control NOD mice, assisting researchers in conveniently and accurately tracing the process of islet loss and further investigating the islet-protective effects of drugs or genes by optical imaging system. Importantly, expressing the anti-PEG reporter in the pancreatic islet of NOD/pIns-αPEG mice does not affect the islet size, insulin secretion, and the disease progression of type 1 diabetes.

備註

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