METHOD AND APPARATUS FOR IMAGE PROCESSING AND VISUALIZATION FOR ANALYZING CELL KINEMATICS IN CELL CULTURE

專利類型

發明

專利國別 (專利申請國家)

美國

專利申請案號

15/800,047

專利證號

US 10,423,819 B2

專利獲證名稱

METHOD AND APPARATUS FOR IMAGE PROCESSING AND VISUALIZATION FOR ANALYZING CELL KINEMATICS IN CELL CULTURE

專利所屬機關 (申請機關)

中原大學

獲證日期

2019/09/24

技術說明

[0001] Fluorescence microscopy has become a powerful and popular tool to obtain digital images from live cells, which help cell biologists visualize and analyze kinematics of cells and/or cell clusters. However, to analyze cell kinematics (such as, cell motion, reproduction, diffusion, and attachment), a massive number of time-series images would be taken, and therefore, the interpretation of these data is quite tedious and time-consuming. The versatility of fluorescence labeling provides pinpoint specificity and the optical sectioning capability of multidimensional fluorescence has advantages of image multidimensionality. To facilitate the analysis, the fluorescence images are often segmented automatically. Nevertheless, the segmented images are usually of low contrast and poor depth resolutions due to the tight packing of cells. In view of this, various techniques or algorithms have been developed for the automatic segmentation. Although these methods may yield the segmentation of cell nuclei in microscopic images, the detection results are often limited in terms of 3D visualization of cells and/or colonies. In view of the foregoing, there exists a need in the related art to provide a method capable of efficiently evaluating the positions, velocities, and states of cell clusters in the time-lapse fluorescence microscopy image.

備註

本會(收文號1120007559)同意該校112年2月7日原產字第1120000364號函申請終止維護專利(中原大學)

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