利用1,2,3,4,6-五-O-沒食子醯基-D-葡哌喃糖來抑制生物膜形成INHIBITION OF BIOFILM FORMATION BY 1,2,3,4,6-PENTA-O-GALLOYL-D-GLUCOPYRANOSE

專利類型

發明

專利國別 (專利申請國家)

美國

專利申請案號

13/236,387

專利證號

US 9,181,290 B2

專利獲證名稱

利用1,2,3,4,6-五-O-沒食子醯基-D-葡哌喃糖來抑制生物膜形成INHIBITION OF BIOFILM FORMATION BY 1,2,3,4,6-PENTA-O-GALLOYL-D-GLUCOPYRANOSE

專利所屬機關 (申請機關)

長庚大學

獲證日期

2015/11/10

技術說明

本發明闡明一藥物1,2,3,4,6-五沒食子醯葡萄糖 (PGG)可應用於臨床上預防金黃色葡萄球菌形成生物膜。PGG不會抑制金黃色葡萄球菌生長，但會抑制其形成生物膜。把PGG塗層於聚苯乙烯，聚碳酸酯或矽膠等材質表面亦可有效抑制金黃色葡萄球菌生物膜的形成。此外，PGG對人類表皮細胞以及纖維母細胞亦不具毒性。PGG抑制百分之50%金黃色葡萄球菌生物膜形成所需的藥物濃度是3.6 μM，此濃度遠低於其他可抑制金黃色葡萄球菌形成生物膜所需的藥物濃度。根據穿透式電子顯微鏡的觀察，PGG抑制細菌黏附到固體表面以及抑制多醣體黏菌素的合成，此說明PGG抑制金黃色葡萄球菌形成生物膜的可能機制。 This invention illustrates a drug, 1,2,3,4,6-Penta-O-galloyl-β-D-glucopyranose (PGG), which is highly promising for clinical use in preventing biofilm formation by Staphylococcus aureus. This invention demonstrates that PGG does not inhibit the S. aureus growth, yet prevents biofilm formation. PGG also inhibits biofilm formation when coated to the surface of polystyrene, polycarbonate and silicon rubber. Meanwhile, PGG is not toxic to human epithelial and fibroblast cells. Also, PGG has an IB50 value, i.e. the PGG concentration that inhibits 50% biofilm formation, of 3.6 μM. The value is substantially lower than the compounds which are known to inhibit biofilm formation by S. aureus. Scanning electron microscopy results also reveal that PGG inhibits initial attachment of the bacteria to solid surface and the synthesis of polysaccharide intercellular adhesin (PIA), explaining how PGG inhibits biofilm formation.

備註

本會(收文號1110078372)同意該校111年12月27日長庚大字第1110120250號函申請終止維護專利(長庚大學)

連絡單位 (專責單位/部門名稱)

技術移轉中心

連絡電話

03-2118800轉3201

網址