以ＰＣＲ-為主之鼠蟯蟲種類鑑別方法PCR-BASED IDENTIFICATION OF RODENT PINWORM SPECIES

專利類型

發明

專利國別 (專利申請國家)

中華民國

專利申請案號

097120998

專利證號

I 373526

專利獲證名稱

以ＰＣＲ-為主之鼠蟯蟲種類鑑別方法PCR-BASED IDENTIFICATION OF RODENT PINWORM SPECIES

專利所屬機關 (申請機關)

國立中興大學

獲證日期

2012/10/01

技術說明

本發明為提供一種利用特定引子組進行PCR，並以電泳分析PCR產物片段，來鑑定及區別鼠蟯蟲種類(Sypacia obvelata, Syphacia muris and Aspiculuris tetraptera) 之方法。本發明之PCR方法，可進一步配合利用特定限制酵素(AluI or RsaI)切割之限制性片段長度多樣性(RFLP)，來鑑定及區別鼠蟯蟲之種類。本發明更進一步設計出，用於專一性增幅不同鼠蟯蟲種類rDNA之ITS-2的引子組。本方法利用特定引子進行檢測若DNA檢體未有該寄生蟲則不會出現PCR產物。本引子特異性極高無交叉反應。因此在PCR-RFLP法運用此引子可成為鑑定各種囓齒動物蟯虫的最佳工具。 This invention involves the construction of specific primers for the Polymerase Chain Reaction (PCR) followed by analysis of the amplicons to differentiate the morphologically similar pinworms of the common laboratory rodents, such as Syphacia obvelata, Syphacia muris and Aspiculuris tetraptera. The use of restriction endonucleases, AluI or RsaI, further allowed the delineation of the three species through the use of Restriction Fragment Length Polymorphism (RFLP). The constructed species specific primers were designed for unique regions of the ITS-2 of the rDNA of the three species. This approach allowed their specific identification with no amplicons being amplified from heterogeneous DNA samples, and sequencing confirmed the identity of the sequences amplified. Thus, the use of these specific primers along with PCR-RFLP can serve as useful tools for the identification of pinworms in rats, mice and wild rodents.

備註

本部(收文號1060063873)同意該校106年8月10日興產字1064300420號函申請終止維護專利

連絡單位 (專責單位/部門名稱)

技術授權中心

連絡電話

04-22851811

網址