非侵入式低頻超音波腦部治療裝置及其使用方法

專利類型

發明

專利國別 (專利申請國家)

中華民國

專利申請案號

097126210

專利證號

I 382860

專利獲證名稱

非侵入式低頻超音波腦部治療裝置及其使用方法

專利所屬機關 (申請機關)

長庚大學

獲證日期

2013/01/21

技術說明

本研究目的在於提出一簡易裝置，以低頻(28-kHz)非聚焦式超音波穿透頭骨達成非侵入式局部血腦屏障開啟的可能性。在動物腦部爲達到局部而非全面之影響，因此設計一簡易單孔擋塊以控制超音波釋放。在聲壓釋放實驗中，對動物施予頻率28kHz的超音波。超音波釋放前並由靜脈注射超音波對比劑以強化血腦屏障開啟之效果。實驗中使用不同的超音波輸出時間，找出可開啟血腦屏障的臨界參數。在動物犧牲後，再藉由Evans Blue切片標示出血腦屏障開啟的區域，並以HE染色法分析對周圍組織細胞造成之傷害程度。結果顯示此一簡易裝置輸出的低頻超音波可以有效達到開啟血腦屏障之目的。 This study proposed a novel approach to locally disrupt BBB by using a simple non-focused low-frequency (28-kHz) ultrasound device. To examine this, SD rats were anesthesia IV, and ultrasound contrast agent was administered before ultrasound energy excitation to enhance the BBB disruption effect. Different low-frequency ultrasound exposure times were tested to find out the threshold condition of BBB disruption. Blue dye was also administered before animal sacrifice for gross observation of the affected region, and H&E as well as TUNEL staining were performed to evaluate the ultrasound induced brain tissue damage. Results confirmed that low-frequency can serve as a controllable tool for localized BBB-disruption. Keyword：low-frequency ultrasound, blood-brain barrier, ultrasound contrast agent

備註

應為CGU-0262 本部(收文號1080079708)同意該校108年12月11日長庚大字第1080120109號函所報專利終止維護案。

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