體外血腦屏障模型之建立方法

專利類型

發明

專利國別 (專利申請國家)

中華民國

專利申請案號

102111425

專利證號

I 523945

專利獲證名稱

體外血腦屏障模型之建立方法

專利所屬機關 (申請機關)

國立中正大學

獲證日期

2016/03/01

技術說明

本發明係揭露一種體外血腦屏障模型之建立方法，首先以比例1：1、1：2或1：6貼附人腦血管周細胞懸浮液及人類星形細胞懸浮液於一培養皿的濾膜之底面，以種植人腦微血管周細胞與人類星形細胞。接著，再於濾膜之頂面注入人腦微血管內皮細胞懸浮液，以種植人腦微血管內皮細胞。種植完後，將培養皿置於存有細胞培養基之孔盤中，並將此置入二氧化碳培養箱進行培養。經一段時間後，更換細胞培養基為條件化培養基。然後，每經一天即更換此條件化培養基，以培養複數天，進而建立生體外人類血腦屏障，以提高其醫學利用性及參考價值。 tTere have been technologies co-culturing rat brain microvascular endothelial cells and either of rat brain vascular pericytes or rat astrocytes, or co-culturing all of them to construct a BBB model. A BBB model, which simultaneously contains astrocytes and brain vascular pericytes in addition to the brain microvascular endothelial cells, has higher transendothelial electrical resistance (TEER) and lower permeability and is more likely to simulate the blood-brain barrier (BBB) effectively. The BBB model co-culturing the abovementioned three types of cells has been used to test drugs. The coverage ratio of brain vascular pericytes to brain microvascular endothelial cells varies with the physiological and pathological statuses. However, the conventional technologies do not pay attention to the coverage ratio of brain vascular pericytes to brain microvascular endothelial cells. Therefore, the conventional technologies lack a BBB model considering the real physiological status.

備註

本部(收文號1100075930)同意該校110年12月29日中正研發字第1100910947號函申請終止維護專利(中正)

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