蛋白質固定方法Method for Immobilizing Glycoprotein

專利類型

發明

專利國別 (專利申請國家)

美國

專利申請案號

12/490,953

專利證號

US 8,153,387 B2

專利獲證名稱

蛋白質固定方法Method for Immobilizing Glycoprotein

專利所屬機關 (申請機關)

國立清華大學

獲證日期

2012/04/10

技術說明

一種抗體固定方法，包含下列步驟：提供一表面；提供一有機硼酸(Boronic acid)接觸至表面，有機硼酸係以Y-R-B-(OH)2表示，其中 (B-(OH)2)為一硼酸基、R為一交聯劑 (linker)、以及Y為一官能基，其中有機硼酸藉由官能基Y結合至表面；以及提供一抗體接觸到有機硼酸，其中抗體之一醣鏈上之任二空間上相近之醇基(diol)會與有機硼酸之硼酸基形成一有機硼酸酯(boronate ester)。本發明藉由有機硼酸與抗體形成位向專一且共價性之方式固定抗體，並且不會破壞抗體之抗原決定基結合能力。 A method for immobilizing antibody includes steps of providing a surface; contacting a boronic acid to the surface, wherein the boronic acid is represented as Y-R-B-(OH)2 and includes a boric acid group(B-(OH)2), a linker R, and a functional group Y, in which the boronic acid is bonded to the surface via the functional group Y; and contacting an antibody to the boronic acid, wherein two hydroxyl groups of a sugar chain of the antibody close to each other in space forms a cyclic boronate ester with the boric acid group of the boronic acid. The goal of site-specific and covalent antibody immobilization without interfering the binding affinity of antibody to epitopes is thus achieved with the reaction of boronic acid and antibody according to the present invention.

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