金屬氧化奈米複合磁性材料為探針之快速萃取分離磷酸化物種分析法 METAL OXIDE NANO-COMPOSITE MAGNETIC MATERIAL, FABRICATION METHOD, AND METHOD FOR LINKAGE, ENRICHMENT, AND ISOLATION OF PHOSPHORYLATED SPECIES

專利類型

發明

專利國別 (專利申請國家)

美國

專利申請案號

12/101,451

專利證號

US 8,735,176 B2

專利獲證名稱

金屬氧化奈米複合磁性材料為探針之快速萃取分離磷酸化物種分析法 METAL OXIDE NANO-COMPOSITE MAGNETIC MATERIAL, FABRICATION METHOD, AND METHOD FOR LINKAGE, ENRICHMENT, AND ISOLATION OF PHOSPHORYLATED SPECIES

專利所屬機關 (申請機關)

國立交通大學

獲證日期

2014/05/27

技術說明

本發明係指一種以金屬氧化物磁性奈米複合材料做為快速濃縮複雜樣品中的磷酸化胜肽及蛋白質的探針的方法。利用奈米複合材料表面包覆之金屬氧化物與磷酸根之間的親和力，並結合其具有磁性可以外加磁場快速分離的特點，由於氧化鐵奈米粒子具有吸收微波的功能，可置於微波爐中加速分析物之濃縮萃取。本發明可以在30~90秒之內成功地濃縮複雜樣品中的磷酸化胜肽及蛋白質，並且可以直接以MALDI MS進行質譜分析，分析一個樣品的時間只需要約5分鐘，並且沒有非特異性吸附的現象產生。相較於目前廣泛使用的其它分離純化方法，本發明具有樣品體積需求量少、專一性高、靈敏度高及快速分析的優點。 This invention describes an approach for rapid enrichment of phosphopeptides and phosphoproteins in complex samples using the metal-oxide nanocomposite magnetic particles. Metal oxides have been known having a high affinity for the phosphate groups. On the basis of the magnetic property of the affinity probes, it could be rapidly isolated from sample solutions by positioning an external magnetic field. Furthermore, iron oxide magnetic nanoparticles have the capacity of absorbing microwave radiation. With addition of the magnetic nanoparticles coated with metal oxide in the sample solution, the enrichment for phosphorylated species could be accelerated as a consequence of the assistance of the magnetic beads in rapid-heating under microwave irradiation. The approach provides high specificity, high sensitivity and rapid analysis for the enrichment of phosphorylated peptides and proteins from complex sample solutions.

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