檢測豬隻早期懷孕之方法及試劑

專利類型

發明

專利國別 (專利申請國家)

中華民國

專利申請案號

103139738

專利證號

I 525323

專利獲證名稱

檢測豬隻早期懷孕之方法及試劑

專利所屬機關 (申請機關)

國立高雄大學

獲證日期

2016/03/11

技術說明

如何正確診斷母豬懷孕與否是影響飼主經營成效的重要因素之一，母豬配種後若到分娩前才發現是空胎或假性懷孕，會增加飼養成本的負擔，減少豬場仔豬年產量，造成農民的經濟損失。因此，亟需要研發出一種更佳的豬隻早期懷孕檢測方法以解決上述問題。本發明係有關於一種檢測豬隻早期懷孕之方法及試劑，檢驗試劑係包括一噬菌體，一抗噬菌體抗體，以及一連接有一標記物之第二抗體，其中噬菌體係表現專一性結合早期懷孕因子(EPF)之胜肽；檢測方法係包括提供一檢體與上述檢驗試劑進行反應，並檢測胜肽是否與早期懷孕因子(EPF)相結合，其若呈免疫陽性反應，則代表檢體具有早期懷孕因子，藉以判斷為懷孕。 The precise diagnosis of sow pregnancy is one of crucial factors which will influence the running effect of a farm. If empty fetus or pseudo-pregnancy was found until mated sow delivery, it would increase the breeding cost and reduce the annual yield of piglet of the farm, and lead to the economic loss of farmer. Hence, it is imperative to have an improved method for detecting sow early pregnancy accurately. The invention relates to a method and a reagent for sow early pregnancy detection. The reagent includes a phage which displays a peptide specific binding to an early pregnancy factor (EPF), a phage antibody specific to the phage, and a secondary antibody specific to the phage antibody. The method includes providing a sample and the reagent for a reaction, and detecting whether the peptide of the phage binds to the early pregnancy factor. If the reaction shows an immune-positive response, it indicates that the sample expresses the early pregnancy factor and is pregnant.

備註

本部(發文號1110013672)同意貴校111年3月9日高大研發字第1111400060號函申請終止維護專利。

連絡單位 (專責單位/部門名稱)

研究發展處

連絡電話

07-5919100