含硫化合物之醫藥用途PHARMACEUTICAL USES OF SULFUR-CONTAINING COMPOUND

專利類型

發明

專利國別 (專利申請國家)

美國

專利申請案號

14/327,482

專利證號

US 9,238,620 B1

專利獲證名稱

含硫化合物之醫藥用途PHARMACEUTICAL USES OF SULFUR-CONTAINING COMPOUND

專利所屬機關 (申請機關)

國立中山大學

獲證日期

2016/01/19

技術說明

Dihydroaustrasulfone alcohol是從海洋珊瑚被分離純化出的化合物，過去研究已經顯示出抗氧化活性，但還沒有被報導具有抗癌作用。我們首次發現dihydroaustrasulfone alcohol 藉由trans-well 和 wound healing assays方式提供濃度依存性對人類非小細胞肺癌（NSCLC）A549 細胞的抑制細胞轉移效果。Zymography assay和Western blot檢測結果顯示 dihydroaustrasulfone alcohol 具抑制基質金屬蛋白酶（MMP）-2和MMP-9蛋白的表現。進一步調查顯示，dihydroaustrasulfone alcohol 能有效抑制 ERK1/2 的磷酸化、P38 和 JNK1/2。 Dihydroaustrasulfone alcohol 也具抑制 PI3K 的表現和 Akt 的磷酸化。此外，dihydroaustrasulfone alcohol 在 Lewis lung cancer (LLC)-bearing mice 實驗中對腫瘤生長有顯著的抑制效果。 Dihydroaustrasulfone alcohol, which is isolated from marine coral, has shown antioxidant activity, but has not been reported to have an anti-cancer effect. We first discovered that dihydroaustrasulfone alcohol provided a concentration-dependent inhibitory effect on the migration and motility of human non-small cell lung carcinoma (NSCLC) A549 cells by trans-well and wound healing assays. The results of a zymography assay and Western blot showed that dihydroaustrasulfone alcohol suppressed the activities and protein expression of matrix metalloproteinase (MMP)-2 and MMP-9. Further investigation revealed that dihydroaustrasulfone alcohol suppressed the phosphorylation of ERK1/2, p38, and JNK1/2. Dihydroaustrasulfone alcohol also suppressed the expression of PI3K and the phosphorylation of Akt. Furthermore, dihydroaustrasulfone alcohol markedly inhibited tumor growth in Lewis lung cancer (LLC)-bearing mice.

備註

本會(收文號1120021252)同意該校112年4月13日中產營字第1121400359號函申請終止維護專利(國立中山大學)

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