利用側向介電泳分離粒子之方法、其流道裝置以及捕捉結構

專利類型

發明

專利國別 (專利申請國家)

中華民國

專利申請案號

102135724

專利證號

I 524930

專利獲證名稱

利用側向介電泳分離粒子之方法、其流道裝置以及捕捉結構

專利所屬機關 (申請機關)

財團法人國家實驗研究院

獲證日期

2016/03/11

技術說明

An antibody-free approach for high purity and high throughput isolation of cancer cells from human blood in a microfluidic chip is presented. High throughput continuous cancer cell isolation (>1.2 ml/hr) is achieved with a sustained dielectrophoresis (DEP) particle force normal to the continuous through-flow. The design allows continuous fractionation of cells into different downstream sub-channels based on the differences in their different critical DEP strengths/mobility on both sides of the cross-over. An enrichment factor of ~10^5 and a recovery rate of ~80% from a 0.001% CTC sample is achieved at a high throughput of 1.2 ml/hr. Compared to conventional physical methods, such as immunomagnetics-based methods and microfluidic biomarker-based methods, the isolation purity, recovery rate, throughput and viability of the proposed antibody-free chip demonstrated excellent capabilities, especially with regard to high enrichment at a high flow rate.

備註

連絡單位 (專責單位/部門名稱)

國研院技術移轉中心

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02-66300686

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