Photoelectrical Device for Concentration Detection, Method for Concentration Detection Thereof and Method for Testing An Antibiotic Susceptibility on Bacteria

專利類型

發明

專利國別 (專利申請國家)

美國

專利申請案號

16/234,597

專利證號

US 11,143,585 B2

專利獲證名稱

Photoelectrical Device for Concentration Detection, Method for Concentration Detection Thereof and Method for Testing An Antibiotic Susceptibility on Bacteria

專利所屬機關 (申請機關)

財團法人國家實驗研究院

獲證日期

2021/10/12

技術說明

An antibody-free approach for high purity and high throughput isolation of cancer cells from human blood in a microfluidic chip is presented. High throughput continuous cancer cell isolation (>1.2 ml/hr) is achieved with a sustained dielectrophoresis (DEP) particle force normal to the continuous through-flow. The design allows continuous fractionation of cells into different downstream sub-channels based on the differences in their different critical DEP strengths/mobility on both sides of the cross-over. An enrichment factor of ~10^5 and a recovery rate of ~80% from a 0.001% CTC sample is achieved at a high throughput of 1.2 ml/hr. Compared to conventional physical methods, such as immunomagnetics-based methods and microfluidic biomarker-based methods, the isolation purity, recovery rate, throughput and viability of the proposed antibody-free chip demonstrated excellent capabilities, especially with regard to high enrichment at a high flow rate.

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國研院技術移轉中心

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02-66300686

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