Method for preserving proliferation and differentiation potential of mesenchymal stem cells

專利類型

發明

專利國別 (專利申請國家)

美國

專利申請案號

14/796,384

專利證號

US 9,688,956 B2

專利獲證名稱

Method for preserving proliferation and differentiation potential of mesenchymal stem cells

專利所屬機關 (申請機關)

國立成功大學

獲證日期

2017/06/27

技術說明

本發明提供一種用於保持未分化細胞之增殖以及分化潛力之方法,其具有下列步驟:提供一具有一表面之培養載體,其中該表面係塗布有一選自於多醣、經硫酸化多醣以及它們的衍生物之生物材料;以及使用一適當的培養基將該未分化細胞殖種並培養於該培養載體的表面上,藉此未分化細胞之增殖以及分化潛力得以被保持。此方法可以被用於活體外擴增幹細胞而不會喪失它們的複製能力以及分化能力。因此,本發明之方法可以被應用於再生醫學、組織工程以及使用臍帶血或其他來源之細胞(諸如週邊血細胞、幹細胞、組織前趨細胞以及組織細胞等)之治療。 A method for preserving proliferation and differentiation potential of undifferentiated cells, has steps of providing a culture carrier having a surface coated with a biological material selected from the group consisting of polysaccharide, sulfated polysaccharide and derivatives thereof; and inoculating and culturing the undifferentiated cells on the surface in the culture carrier with an appropriate medium, such that the proliferation and differentiation potential of undifferentiated cells are preserved. The method can be used for expanding stem cells in vitro without loss of their replicative ability and differentiation capacity. Therefore, the method according to the present invention is amenable to application in regenerative medicine, tissue engineering, and therapy using umbilical cord blood and other cell sources such as peripheral blood, stem cells, tissue progenitor cells, and tissue cells.

備註

連絡單位 (專責單位/部門名稱)

企業關係與技轉中心

連絡電話

06-2360524

網址