METHODE DE TRAITEMENT D'UNE INFECTION VIRALE ET PREPARATION DE COMPOSITIONS ANTIVIRALES

專利類型

發明

專利國別 (專利申請國家)

加拿大

專利申請案號

CA2984928

專利證號

2984928

專利獲證名稱

METHODE DE TRAITEMENT D'UNE INFECTION VIRALE ET PREPARATION DE COMPOSITIONS ANTIVIRALES

專利所屬機關 (申請機關)

國立臺灣大學

獲證日期

2021/03/16

技術說明

EV71 causes many fatal cases worldwide and adenoviruses are highly transmissible with great disease burden in children, but there is no available commercial antiviral medication against adenoviruses or enterovirus 71. Therefore, we screened antiviral activities of plants and herbs with different methods of extraction and different combinations. We measured the IC50 (50% inhibitory concentration) against adenovirus type 3 and enterovirus 71 (EV71) by plaque assay, CC50 (50% cytotoxicity concentration) by XTT cytotoxicity assay and calculated the selective index (SI: CC50/IC50). According to IC50 and SI, 2 best candidates (the water extract of Echinacea purpurea aerial component, EPA-W, for adenovirus serotype 3 with IC50 of 4.6 ug/ml and SI>600; the ethanol extract of fresh Salvia miltiorrhiza, SMf-2-E, for EV71 with IC50 8.3 ug/ml and SI: >100) were chosen to make this antiviral pharmaceutical combination. The optimal combination ratio of EPA-W: SMf-2-E was 3:7 to have the best antiviral activity. We further investigated their antiviral mechanism and found that the antiviral mechanism of EPA-W was mainly through inhibiting attachment and that of SMf-2-E was mainly through inhibiting penetration. This antiviral pharmaceutical combination of EPA-W and SMf-2-E, safe and effective with different antiviral mechanism, can be applied to make antiviral therapeutic or preventive products in the future.

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