Method for bioconversion of mogroside extracts into siamenoside I

專利類型

發明

專利國別 (專利申請國家)

美國

專利申請案號

16/584,216

專利證號

US 11,180,789 B2

專利獲證名稱

Method for bioconversion of mogroside extracts into siamenoside I

專利所屬機關 (申請機關)

國立臺灣大學

獲證日期

2021/11/23

技術說明

本研究團隊藉由篩選不同微生物對羅漢果皂苷水解之能力(科技部計畫105-2320-B-002-026-MY3)結果中發現酒香酵母(Dekkera bruxellensis)中之特定酵素可生產羅漢果皂苷田味劑Siamenoside I(羅漢果皂苷中目前甜度最高之皂苷)(Food Chem.,2019)。此研究為目前文獻中唯一一篇找到可以特定酵素產出siamenoside I。研究中指出因原菌株生長速度相當緩慢，因此藉由酵母菌基因工程的方式進行改善，目前可產出兩株具有大量表現此酵素且可快速轉換羅漢果皂苷粗萃物並生成siamenoside I之代糖。 Our team has investigated microbial bioconversion of mogrisdes for many years. The projected was also funded by MOST (105-2320-B-002-026-MY3). Our results demonstrated that a specific enzyme from Dekkera bruxellensis can biotransform mogrosides extract and produce Siamenoside I, the sweetest form of mogrosides (Food Chem.,2019). In addition, the original strain grew slow and was not efficient to bioconvert mogroside extract. We then genetically engineered two new strains to over express the specific gene, encoding the functional protein to bioconvert the mogrosides into siamenoside I products.

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