經雜環接枝之聚醯胺酸及用於電化學裝置之電極POLYAMIC ACID AND ELECTRODE FOR DETECTING HYDROGEN PEROXIDE

專利類型

發明

專利國別 (專利申請國家)

美國

專利申請案號

13/445,140

專利證號

US 8,858,767 B2

專利獲證名稱

經雜環接枝之聚醯胺酸及用於電化學裝置之電極POLYAMIC ACID AND ELECTRODE FOR DETECTING HYDROGEN PEROXIDE

專利所屬機關 (申請機關)

長庚大學

獲證日期

2014/10/14

技術說明

本發明是以聚醯胺酸苯並惡唑或聚醯胺苯並噻唑經修飾金電極後做為感測電極，在磷酸鹽緩衝溶液中能在負電位下偵測過氧化氫。由於聚醯胺酸苯並惡唑或聚醯胺苯並噻唑具有亞胺與羧酸基團，修飾金電極後即可偵測過氧化氫，其中，聚醯胺酸苯並惡唑經在非溶劑下奈米化後，能明顯的提升其過氧化氫感測器之表現。再針對膀胱癌患者病人尿液檢測中，證實其過氧化氫濃度均高於正常人，與標準過氧化氫檢驗方式比較，本發明所提出之修飾電極所得到的結果在統計學上為無誤差，顯示其修飾電極具有極高之選擇性，可避免尿液中其他物質之干擾，呈現出準確之結果。本發明製備的修飾電極能以塗佈或沉浸的方式在 50 ℃ 減壓乾燥條件下快速完成，製程簡單便宜，由於製備出的複合材料成膜性、耐熱性與環境安定性佳，在感測元件的保存上有很大的突破。 Polyamic acids (PAAs) containing benzothiazole (BT) and benzoxazole (BO) pendent groups (PAA-BT and PAA-BO, respectively) which possessed electroactivity were synthesized successfully. These biosensors had rapid response times (3.9~5.2 s) and high selectivity and sensitivity (280.6 ~ 311.2 μA/mM-cm2). Modifying the surface morphology of the electrode from a planar to a three-dimensional (3D) configuration enhanced the performance of the PAA-BO/Au electrode. The sensitivity of the 3D-PAA-BO/Au electrode was 1394.9 mA/mM-cm2, ~ 4.5 times higher than that of the planar electrode. The detection limit was also enhanced from 5.0 to 1.43 μM. The results were promising and comparable to that measured by a classical HPLC method, which verified the developed biosensor had a potential to provide a usefully analytical approach for bladder cancer.

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