晶片式微流體粒子三維聚焦及偵測裝置

專利類型

發明

專利國別 (專利申請國家)

中華民國

專利申請案號

092113698

專利證號

198083

專利獲證名稱

晶片式微流體粒子三維聚焦及偵測裝置

專利所屬機關 (申請機關)

國立成功大學

獲證日期

2004/07/07

技術說明

本發明係提供一種晶片式微流體三維粒子聚焦及偵測裝置，該 裝置利用流體力（hydrodynamic force）以及介電泳力 （dieletrophoretic force）之作用，使微粒子得以流動在微 管道之中央，以增加後續偵測之準確性。本發明之晶片乃先在 基板上製作出提供流體流動之微管道，包括樣品流管道以及邊 鞘流管道，對樣品流中之粒子進行二維之流體聚焦，並在微管 道中製作微型電極，以提供介電泳力進行第三維之粒子聚焦。 本發明可應用於各種微粒子如：細胞、血球等之計數檢測、判 別、測速、分類等測量。 This invention reports a micro flow cytometer with innovative 3-dimensional focusing function that can focus cells in the center of sample stream, resulting in a high-precision cell counting. A novel method is used to focus the cells 3-dimensionally using the combination of hydrodynamic forces and dielectrophoretic forces. Focusing of the sample flow is a critical issue for micro flow cytometers while counting the particles/cells using a buried optical waveguide. Appreciable errors could occur if the particles are randomly distributed vertically even though they have been focused horizontally. In this paper, a 3-D focusing flow cytometer was demonstrated using MEMS fabrication techniques. Three major modules have been integrated on glass substrates to form the microfluidic device, including a hydrodynamically- driven flow cytometer, microelectrode arrays for dielectrophoretic forces and etched optical fibers for on-line cell detection.

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