用於抑制α-葡萄糖苷酶之組合物

專利類型

發明

專利國別 (專利申請國家)

中華民國

專利申請案號

106112723

專利證號

I 615142

專利獲證名稱

用於抑制α-葡萄糖苷酶之組合物

專利所屬機關 (申請機關)

淡江大學學校財團法人淡江大學

獲證日期

2018/02/21

技術說明

先期研究成果(已向我國智慧財產局提出新發明專利申請)，自台灣土壤篩選出BCRC910751這株經鑑定為Paenibacillus sp.的類芽孢桿菌屬細菌。無論是用市售細菌培養營養液(nutrient broth)或是實驗室自調之培養液(1%蝦頭殼粉、0.l % K2HPO4、0.05 % MgSO4．7H2O)，所得發酵上清液對於源自大鼠或酵母菌的α-葡萄糖苷酶，都具有足以匹敵要用商品Acarbose之抑制活性。經口餵食老鼠實驗也證實，本發明BCRC910751發酵上清液之糖苷酶抑制劑，具有足以和Acarbose匹敵之血糖控制效果，而且血糖降低速度比Acarbose的穩定。發酵上清液與Acarbose對於老鼠血糖控制，具有顯著之相乘效果。更尤甚者，老鼠服用BCRC910751發酵所得上清液，不會有Acarbose那樣會造成老鼠腹瀉之情形。 本發明利用BCRC910751這株α-葡萄糖苷酶抑制劑生產菌，發酵簡稱NB之培養液，所得上清液之冷凍乾燥物，以Diaion Gel、Silica gel層析分離法，得到五個具有比Acarbose(糖尿病用藥)更強α-葡萄糖苷酶抑制活性之化合物Adenine, 3-hydroxy-dl-proline, (3,6-dioxo-piperazin-2-yl)-acetic acid amide, 2-ethylhexyl heptanoate, 以及nicotinic acid。 The glucosidase inhibitor-producing strain BCRC910751 was isolated from Tamsui soils. After cultivation in nutrient broth, the obtained culture supernatants were purified with Diaion gel and Silica gel column chromatography. Ten compounds were isolated, in which 3 active compounds (3, 5, 9) and a component containing active compounds (7-8) shower stronger inhibitory activities than that of acarbose (a commercial anti diabetes drug). Using spectroscopic analyses and comparisons, these compounds (3, 5, 7, 8 and 9) were identified as Adenine, 3-hydroxy-dl-proline, (3,6-dioxo-piperazin-2-yl)-acetic acid amide, 2-ethylhexyl heptanoate, and Nicotinic acid, respectively.

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