有機相容質甜菜鹼之活體外及活體內生合成IN VIVO AND IN VITRO BIOSYNTHESIS OF THE OSMOLYTE GLYCINE BETAINE

專利類型

發明

專利國別 (專利申請國家)

中華民國

專利申請案號

097145828

專利證號

I 394835

專利獲證名稱

有機相容質甜菜鹼之活體外及活體內生合成IN VIVO AND IN VITRO BIOSYNTHESIS OF THE OSMOLYTE GLYCINE BETAINE

專利所屬機關 (申請機關)

國立中興大學

獲證日期

2013/05/01

技術說明

本發明係關於，編碼嗜鹽甲烷古菌Methanohalophilus portucalensis FDF1的甜菜鹼生合成酵素肌胺酸二甲基甘胺酸甲基轉移酶 (MpSDMT)與甘胺酸肌胺酸甲基轉移酶 (MpGSMT)之經單離核酸分子，以及利用於選殖Mpgsmt與Mpsdmt基因之引子序列。本發明亦關於，利用重組基因技術將Mpgsmt與Mpsdmt基因單獨或一起，轉殖入大腸桿菌中大量表現MpGSMT與/或MpSDMT重組蛋白的方法。本發明更進而提供，利用該二種具甲基轉移功能的MpGSMT與MpSDMT蛋白，以活體內或活體外方式將甘胺酸轉化生成肌胺酸(sarcosine)、二甲基甘胺酸(dimethylglycine)及甜菜鹼(glycine betaine)這三種有機相容質的方法，以及利用其促進生物對抗環境逆境、提高適應能力之用途。 The inventor- Professor Lai has been demonstrated that the moderate and extreme halophilic methanoarchaea could synthesis glycine betaine as osmolyte by three step methylation of glycine to form sarcosine, dimethylglycine and glycine betaine to survive in the hypersaline environment. Cloning and expression vectors contain Mpgsmt and Mpsdmt separately or together were successfully transformed and expressed at E. coli. This invention provided two methyltransferase gene and proteins which could biosynthesis in vivo or in vitro the osmolyte sarcosine, dimethylglycine or glycine betaine. Moreover, this invention also provide a vector contains glycine betaine biosynthesizing genes which can further transformed to other bacteria, plant and animals and promote the synthesis of osmolyte glycine betaine under salt, osmotic, drought and heat stresses. This green biotechnology could help to protect crops and aquaculture from the extreme environment due to global warming.

備註

本部(收文號1080029338)同意該校108年5月10日興產字第1084300302號函申請終止維護專利

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