分化成均一之神經細胞之方法

專利類型

發明

專利國別 (專利申請國家)

中華民國

專利申請案號

098137002

專利證號

I 373524

專利獲證名稱

分化成均一之神經細胞之方法

專利所屬機關 (申請機關)

國立臺灣師範大學

獲證日期

2012/10/01

技術說明

本發明係揭露一種分化成均ㄧ之神經細胞之方法，其步驟包括：將類胚胎體培養於含有第一培養基之第一培養皿中，再加入誘導劑，使得類胚胎體分化成神經前驅細胞，接著，利用細胞濾器將含有神經前驅細胞之第一培養基過濾，以取得神經前驅細胞。爾後，加入分離劑於神經前驅細胞中，並淹沒神經前驅細胞，使分離劑與神經前驅細胞作用7-9分鐘，進而使神經前驅細胞彼此分散，再將分散之神經前驅細胞培養於含有第二培養基之第二培養皿中，使神經前驅細胞分化為均ㄧ之神經細胞。 Much effort is being marshaled to generate uniform neuronal populations from embryonic stem (ES) cells, but a completely reliable method has yet to be developed. Herein, we modified and established a method that brings us closer to this goal. By examining many parameters, we found that the optimal timing of applying a freshly made trypsin/EDTA solution to dissociate embryoid bodies determines the success of the outcome. Analyses demonstrated that with this approach, > 87% of cells differentiated into glutamatergic neurons. Hence, these uniform neurons which were differentiated from ES cells provide an ideal cellular model for many aspects of research.

備註

本部(發文號1100066083)同意貴校110年8月30日師大研合字第1101021150號函申請終止維護專利。(師大)

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產學合作組

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