Direct Measurements of Nanoparticles and Virus by Virus Mass Spectrometry

專利類型

發明

專利國別 (專利申請國家)

美國

專利申請案號

14/274,707

專利證號

US 9,006,650 B2

專利獲證名稱

Direct Measurements of Nanoparticles and Virus by Virus Mass Spectrometry

專利所屬機關 (申請機關)

中央研究院

獲證日期

2015/04/14

技術說明

這個方法和電噴灑法(Electrospray Ionization(ESI)) 來測量生物分子類似、主要不同的地方有三:(1) 雷射導致超音波脫出法用來將奈米粒子或病毒拋進質譜儀中、不是用介質輔助雷射拋出法(Matriz-assisted laser desorption/ionization (MALDI)) 或電噴灑法(2) 質荷比分析儀可以測量質荷比達到百萬以上、所有商業質譜儀沒有此種功能 (3) 直接測量總電荷數的能力、商業質譜儀用電荷放大測量器、但這種測量器不適合測量質荷比高的奈米粒子或病毒。 我們用這方法己成功地測量出愛滋病毒、不同的流感病毒及不同體積的奈米粒子的質量、這將是對病毒鑑定和奈米粒子生產的品質管控做出重大的貢獻。 The major differences between our approach and conventional ESI for large biomolecule detection are (1) laser-induced acoustic desorption (LIAD) is used for the desorb the samples while typical ESI is with through the spray process. (2) our mass analyzer has the capability to analyze ions with m/z higher than 108. No commercial mass spectrometers can measure m/z in this region.and (3) direct charge measurement: Nearly all commercial mass spectrometers use charge amplification device such as a channeltron, electromultiplie of microchannel plate (MCT) for detection. A charge amplification device cannot work well with the m/z of the charged particle higher than 106. We have used our new approach to successfully measured the masses of HIV, and different types of flu viruses. This technology should be valuable for virus detection and quality control for nanoparticle productions.

備註

本會(收文號1120050603)同意該校112年7月31日智財字第1121702144號函申請終止維護專利(中央研究院)

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