METHOD OF SCREENING, ISOLATING,AND PURIFYING RARE CELLS

專利類型

發明

專利國別 (專利申請國家)

美國

專利申請案號

15/817,431

專利證號

US 10,578,607 B2

專利獲證名稱

METHOD OF SCREENING, ISOLATING,AND PURIFYING RARE CELLS

專利所屬機關 (申請機關)

長庚大學

獲證日期

2020/03/03

技術說明

時至今日，癌症佔據台灣國民十大死因的首位已有十餘年之久。儘管對癌症的治療已有了極大的進展，但診治上仍受限於癌症進程、轉移與抗藥性生成之快速，從而無法有效地根除人體內的癌細胞。此外，臨床之癌症診斷有賴於組織切片與生物標記之鑑定，但這些方法都無法即時反應腫瘤組織的真實情況，更不提組織採樣手法複雜且易影響病人術後之康復時間。有鑑於前端檢測之重要性，目前諸多研究建議使用血液中的循環腫瘤細胞團塊作為診斷標的，其在動物實驗上已被證實與癌症轉移能力成正相關，且關聯性遠大於單一循環腫瘤細胞。本發明主要是針對血液中循環腫瘤細胞團塊進行收集，從而提供一個能高度分離且排除其他血球細胞干擾之純化平台。藉由 Ficoll 技術去除大多數的紅血球後，將剩餘細胞通入光誘導介電泳力晶片中，以液體流動與光學虛擬微型過濾裝置來區別循環腫瘤細胞團塊與其他血球細胞，並運輸至收集槽，可達到自動純化之目的。透過此平台收集到之循環腫瘤細胞團塊，便可由其數量推估病人之癌症進程、預後情況與轉移之可能性，同時配合 qPCR、NGS 等基因辨識技術則可預測癌症抗藥性之出現，有助於臨床治療策略之轉換。 Cancer has remained the first rank of ten leading death causes in Taiwan for decades. Although cancer treatments have advanced significantly, it has been restricted in the rapid cancer progression, metastasis and drug resistance. Based on the importance of early detection, many studies have suggested circulating tumor cell clusters (CTC clusters) could be applied as targets for clinical diagnosis. This invention is focused on CTC clusters collection in the blood. Using the Ficoll technique to remove red blood cells, the rests of cells were injected into an optically-induced dielectrophoretic chips which separated of CTC clusters and other cells by liquid flows and optically-virtual microfiltration devices. Owing to purification of this platform, the possibility in cancer progression, prognosis and metastasis of patients can be estimated by the number of CTC clusters. Meanwhile, using gene analysis techniques can predict the appearance of cancer drug resistance.

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