包埋微生物膠體、其製備方法以及降解含氯有機物的組合物

專利類型

發明

專利國別 (專利申請國家)

中華民國

專利申請案號

108114190

專利證號

I 701324

專利獲證名稱

包埋微生物膠體、其製備方法以及降解含氯有機物的組合物

專利所屬機關 (申請機關)

國立中央大學

獲證日期

2020/08/11

技術說明

本案欲自現地地下水中篩選出產氫菌株，並利用固定化技術將產氫菌株包埋於膠體之中，以增加產氫菌株產氫之穩定度、產氫活性與毒物耐受性，進而提升野外地下水含氯有機物整治效益。本案提供一種微生物，自污染場址地下水中篩選出具有產氫氣能力的菌種丁酸梭菌(Clostridium butyricum)，可在三氯乙烯在還原脫氯的過程中，提供氫氣作為電子之供應者，以此協助環境脫氯菌加速降解含氯有機物。前人研究指出氫氣在厭氧還原脫率過程中扮演重要角色，而地下水現地微生物中產氫菌株由於競爭、碳源不足等原因，常無法穩定提供氫氣做為脫氯菌群還原脫氯電子來源，導致還原脫氯效率低落，而菌株包埋化技術能強化菌株耐受性與穩定性。本案選用矽膠體作為包埋載體，此膠體可以通透有機物，使得被包埋之菌種可以有效利用環境基質，穩定產出氫氣。將本案所製之膠體用於地下水環境行生物復育，至少於30天內能夠穩定產出氫氣，經由添加包埋菌可以提升環境降解含氯有機物之速率。 In this case, the hydrogen-producing strains were selected from the groundwater, and the hydrogen-producing strains were embedded in the colloid by immobilization technology to increase the hydrogen production stability, hydrogen production activity and toxic tolerance of the hydrogen-producing strains, thereby enhancing the field. The benefits of remediation of chlorine-containing organic matter in groundwater. The present invention provides a microorganism, which screens out the hydrogen-producing strain Clostridium butyricum from the contaminated site groundwater, and provides hydrogen as a supplier of electrons in the process of reductive dechlorination of trichloroethylene. This helps the environmental dechlorination bacteria to accelerate the degradation of chlorine-containing organic matter. Previous studies have pointed out that hydrogen plays an important role in the process of anaerobic reduction and depletion. However, due to competition and insufficient carbon sources, hydrogen-producing strains in groundwater are often unable to provide hydrogen as a source of dechlorination electrons for dechlorination. The reduction of dechlorination efficiency is low, and the strain embedding technology can enhance the tolerance and stability of the strain. In this case, the colloidal colloid is used as the embedding carrier, and the colloid can penetrate the organic matter so that the embedded species can effectively utilize the environmental substrate and stably produce hydrogen. The colloid prepared in this case is used for biological re-cultivation in the groundwater environment, and the hydrogen can be stably produced in at least 30 days, and the rate of environmental degradation of the chlorine-containing organic matter can be improved by adding the embedded bacteria.

備註

本會(收文號1120018507)同意該校112年3月30日中大研產字第1121400353號函申請專利讓與(國立中央大學)

連絡單位 (專責單位/部門名稱)

智權技轉組

連絡電話

03-4227151轉27076

網址