用以評估腫瘤增生、侵犯或轉移風險之生物標記及方法BIOMARKER AND METHOD FOR EVALUATING RISK OF PROLIFERATION, INVASION, OR METASTASIS OF CANCER

專利類型

發明

專利國別 (專利申請國家)

中華民國

專利申請案號

100124616

專利證號

I 498564

專利獲證名稱

用以評估腫瘤增生、侵犯或轉移風險之生物標記及方法BIOMARKER AND METHOD FOR EVALUATING RISK OF PROLIFERATION, INVASION, OR METASTASIS OF CANCER

專利所屬機關 (申請機關)

國立成功大學

獲證日期

2015/09/01

技術說明

本案用於偵測肝細胞癌的癌症標記蛋白是一個大腦組織特異表現蛋白，經研究發現該蛋白只易位性表現於肝癌細胞中但不表現於正常的肝組職及良性的肝臟血管瘤中，此外在轉移到其他部位的肝癌細胞檢體中也可偵測到此蛋白的表現。細胞實驗證實過量表現此蛋白於癌化程度較低的低去分化(low dedifferentiation)肝癌細胞株HepG2中，會促使細胞轉化為具有高增生能力及高侵犯能力的細胞，動物實驗證明，異體移植之過量表現TIAM2的HepG2細胞株所衍生出的腫瘤，會侵入鄰近腫瘤的裸鼠肌肉組織。基於對此蛋白在肝癌細胞中表現的分析和細胞內功能性研究結果顯示，此蛋白易位性表現的情形廣泛被偵測存在於肝癌細胞中並參與肝癌癌化過程，基於研究結果顯示此蛋白可作為一個新的肝癌標記，經由蛋白表現的測定可提高肝癌診斷的準確率降低偽陽性，預期可幫助臨床診斷並作為術後治療及預後評估之參考指標。 This protein is a brain-specific expression protein. We had previously demonstrated this protein ectopic expressed in Hepotocellular carcinoma (HCC) but not in normal liver tissue and benign hemangioma. In addition, the protein is detected in matastatic parts of HCC. Stable expression of recombinant protein promoted growth and invasive ability in HepG2 cells (a low dedifferentiated HCC cell line). To test tumorigenicity of this protein in vivo, xenograft tumor raised from stable clones invaded into surrounding muscle tissue of nu mice. Based on screening of expression pattern in HCC and functional assay for this protein, it revealed that ectopic expression of this protein was commonly existed in HCC and involved in carcinogenesis of HCC. It is suggested that the protein is a novel cancer marker of HCC. By the usage of this protein as a marker, which could improve the accuracy of HCC diagnosis, and it is provided an indicator for cure of post-surgery and evaluation of prognosis.

備註

本部(收文號1080026227)同意該校108年4月26日成大研總字第1081103516號函申請終止維護專利

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