用於鑑別H5禽流感病毒病原性之寡核酸探針、含其之套組及方法 OLIGONUCLEOTIDE PROBES, KIT CONTAINING THE SAME AND METHOD FOR PATHOTYPING OF H5 AVIAN INFLUENZA VIRUSES

專利類型

發明

專利國別 (專利申請國家)

美國

專利申請案號

14/286,402

專利證號

US 10,017,830 B2

專利獲證名稱

用於鑑別H5禽流感病毒病原性之寡核酸探針、含其之套組及方法 OLIGONUCLEOTIDE PROBES, KIT CONTAINING THE SAME AND METHOD FOR PATHOTYPING OF H5 AVIAN INFLUENZA VIRUSES

專利所屬機關 (申請機關)

國立臺灣大學

獲證日期

2018/07/10

技術說明

禽流感H5亞型病毒造成養雞業很大的經濟衝擊。禽流感高、低病原性的快速診斷與正確鑑別是必須的，特別是針對H5亞型病毒的爆發與監控時。我們發明的計術可以對H5亞型病毒作快速病原分型。利用寡核甘酸微陣列，H5病毒基因血液凝集素前驅蛋白基因切割位的鹼性胺基酸數目可以被偵測出來。運用13個所設計的微陣列探針，高與低病原性的禽流感病毒可以用肉眼鑑別出。其偵測的極限可達3.4病毒RNA複製數，此為傳統反轉錄PCR方法的1000倍。由此，本發明的技術可提供缺乏相關貴重實驗設備的實驗室的另一種檢測選擇。 The H5 avian influenza virus subtype has huge impact on the poultry industry. Rapid diagnosis and accurate identification of the highly pathogenic avian influenza virus and low-pathogenicity avian influenza virus is essential, especially during H5 outbreaks and surveillance. To this end, a novel and rapid strategy for the H5 virus molecular pathotyping is presented. The specific hemagglutinin gene of the H5 virus and the basic amino acid number of the motif at the hemmagglutinin precursor protein cleavage site were detected using oligonucleotide microarray. Highly pathogenic and low-pathogenicity avian influenza viruses in Taiwan were differentiated using 13 microarray probes with the naked eyes. The detection limit reached 3.4 viral RNA copies, 1,000 times more sensitive than reverse transcription polymerase chain reaction. Thus, the oligonucleotide microarray would provide an alternative H5 pathogenicity determination using naked eye for laboratories lacking facilities.

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