新穎的紫色非硫光合細菌及其應用與篩選方法

專利類型

發明

專利國別 (專利申請國家)

中華民國

專利申請案號

101136842

專利證號

I 464260

專利獲證名稱

新穎的紫色非硫光合細菌及其應用與篩選方法

專利所屬機關 (申請機關)

國立臺灣大學

獲證日期

2014/12/11

技術說明

本發明係有關於一種由台灣大學水田土壤中所分離純化培養之微生物株，特別是一種具有(1)生物固氮(biological nitrogen fixation)、(2) C4 酯解酶 (esterase)、(3) C8 酯解脂解酶(esterase lipase)、(4) 醯胺酶（leucine arylamidase）(5) 胱氨酸氨基肽酶（Cystine arylamidase）、(6) 胰蛋白酶 （trypsin）、(7) 磷酸水解酶 (phosphatase)、(8)萘酚-AS-BI-磷酸水解酶（Naphthol-AS-BI-phosphohydrolase ）的活性、(9)可產生植物生長激素－吲哚－3－乙酸 (indole－3－acetic acid；IAA)等至少9種功能之紫色非硫光合菌 (Rhodopseudomonas palustris NTUIOB-PS3) 。 A novel bacterial strain, designated NTUIOB-PS3, isolated from paddy soil of experimental field at the National Taiwan University in Taiwan, was characterized. Strain NTUIOB-PS3 was rod-shaped, Gram-negative, pale-yellow-pigmented in aerobic and red-pigmented in anaerobic conditions. It grew optimally at 25–37 °C, in the presence of 0.75% (w/v) NaCl and pH 5.0-9.0. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain belonged to the species Rhodopseudomonas palustris. The following characteristics were present in the strains : biological nitrogen fixation, C4 esterase, leucine arylamidase, Cystine arylamidase, trypsin, phosphatase, Naphthol-AS-BI-phosphohydrolase and IAA (indole－3－acetic acid) production ability.

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