多目標引子對設計方法

專利類型

發明

專利國別 (專利申請國家)

中華民國

專利申請案號

109146308

專利證號

I 760998

專利獲證名稱

多目標引子對設計方法

專利所屬機關 (申請機關)

國立高雄科技大學

獲證日期

2022/04/11

技術說明

A method for designing a multi-objective primer pair is disclosed. The method includes inputting a DNA template fragment, a length of a forward primer, a length of a reverse primer, at least two objectives and optimal values for each of the at least two objectives to a computer system; generating, by the computer system, a plurality of primer pairs according to the DNA template fragment, the length of the forward primer and the length of the reverse primer; and calculating, by the computer system, numerical values of the at least two objectives of each of the plurality of primer pairs and inputting the numerical values of the at least two objectives of each of the plurality of primer pairs to a Pareto Chart tool to obtain at least one primer pair, and taking the primer pair as an optimal solution of the DNA template fragment.

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