一種基因檢驗方法METHODS AND KITS FOR THE DETECTION OF NUCLEOTIDE MUTATIONS USING PEPTIDE NUCLEIC ACID AS BOTH PCR CLAMP AND SENSOR PROBE

專利類型

發明

專利國別 (專利申請國家)

美國

專利申請案號

11/624,796

專利證號

US 7,803,543 B2

專利獲證名稱

一種基因檢驗方法METHODS AND KITS FOR THE DETECTION OF NUCLEOTIDE MUTATIONS USING PEPTIDE NUCLEIC ACID AS BOTH PCR CLAMP AND SENSOR PROBE

專利所屬機關 (申請機關)

長庚大學

獲證日期

2010/09/28

技術說明

N/A Disclosed herein is a method for determining whether a target polynucleotide sequence contained in a nucleic acid sample has nucleotide variation(s) in a selected region thereof, the steps of which involve the use of a pair of primers that allows the formation of a PCR product having a sequence covering that of the selected region of the target polynucleotide sequence via a PCR process, and a peptide nucleic acid(PNA) that acts as a PCR clamp as well as a sensor probe. Also disclosed herein is a kit for use in determining the presence of nucleotide variation(s) in the target polynucleotide sequence, which includes the primers and the PNA.

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