具耐熱性蛋白質疫苗佐劑及其製備方法

專利類型

發明

專利國別 (專利申請國家)

美國

專利申請案號

13/745,086

專利證號

US 8,729,237 B1

專利獲證名稱

具耐熱性蛋白質疫苗佐劑及其製備方法

專利所屬機關 (申請機關)

國立清華大學

獲證日期

2014/05/20

技術說明

介白素-1 beta (IL-1β) 已被廣泛應用於疫苗佐劑，藉以刺激趨化細胞激素的分泌和提升抗體的生產。但其跨物種的生物免疫反應不盡相同，且其佐劑製造過程中IL-1β的穩定度扮演重要的角色。為了有效預防禽類疾病的暴發，我們以蛋白質環狀重組(CP)法，將野生型(wild-type)雞的IL-1β胺基酸序列進行設計改良，並建構出一新型雞的IL-1β，將其命名為CP-IL-1β，我們設計的CP-IL-1β蛋白質對熱的耐受性明顯提升，同時也具備其原本的生物免疫活性，此一新型的CP-IL-1β有助於開發高效能的禽類IL-1β疫苗佐劑並應用於醫療等用途。 Interleukin-1β is mainly as a biological adjuvant, but its instability is limited the application. The present invention provides an improved circular permutation chicken interleukin-1β which is obtained by the combination of structural biology, biological information and genetic engineering. Because there are many protein adjuvants unstable at the temperature above 46°C, and chicken interleukin-1β is also included. The circular permutation chicken interleukin-1β of the present invention has been broken through the barrier which has not been overcome by other technology. Furthermore, the present invention also provides a most suitable condition to manufacture and purify the circular permutation chicken interleukin-1β, which is able to facilitate industrial applications.

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