以基因重組微生物進行碳源轉化生產生物降解高分子與生質燃料的方法Method for producing biodegradable polymer and biomass fuel converted from carbon source by recombinant microorganisms

專利類型

發明

專利國別 (專利申請國家)

美國

專利申請案號

14/301,882

專利證號

US 9,169,501 B2

專利獲證名稱

以基因重組微生物進行碳源轉化生產生物降解高分子與生質燃料的方法Method for producing biodegradable polymer and biomass fuel converted from carbon source by recombinant microorganisms

專利所屬機關 (申請機關)

元智大學

獲證日期

2015/10/27

技術說明

本發明揭露一種以基因重組微生物進行碳源轉化生產生物降解高分子與生質燃料的方法，其步驟包括：(A)以基因重組技術構築具有一粗甘油甘油利用酵素基因及一聚羥基烷酯合成酶基因之基因重組微生物；(B)將該基因重組微生物培養於一含有粗甘油甘油之環境中；(C)誘導該基因重組微生物表現該粗甘油甘油利用酵素及該聚羥基烷酯合成酶並生產聚羥基烷酯之生物降解高分子及乙醇之生質燃料；(D)分離取得該聚羥基烷酯及乙醇；其中該基因重組微生物之細胞內所產生的聚羥基烷酯至少佔其生物量之30 % (w/w) ，且其粗甘油甘油消耗可達90 % ( w/w)以上。 Transformation of recombinant E. coli harbored two plasmids by genetic engineering technology, which contains utilization of crude glycerol aldehyde reductase、aldehyde dehydrogenase and PhaCAB of PHAs synthase. After using crude glycerol as a sole carbon source incubated at 37 ℃, 200 rpm then using L-arabinose and IPTG as inducers added in culture broth. Culture results of recombinant E. coli (dual-plasmid) showed 90 % crude glycerol utilization rate more than wild-type E. coli 50 % utilization, it had nearly 2 folds distance and it had 30 % (w/w) PHAs accumulated content. Comparison of cultured in the aerobic and anaerobic conditions, the performance were cultured in aerobic environment, its efficiency of crude glycerol utilization and PHAs accumulation were significantly better than the anaerobic environment and using wild-type E. coli performance, too.

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