α-突觸核蛋白感測薄膜及其製造方法與用途

專利類型

發明

專利國別 (專利申請國家)

中華民國

專利申請案號

109118820

專利證號

I 745993

專利獲證名稱

α-突觸核蛋白感測薄膜及其製造方法與用途

專利所屬機關 (申請機關)

國立高雄大學

獲證日期

2021/11/11

技術說明

"The present invention relates to an α-synuclein sensing film, a manufacturing method and a use thereof. The α-synuclein sensing film is anufactured by imprinting an α-synuclein peptide on a high molecular weight polymer to obtain the α-synuclein sensing film for detecting α-synuclein in a sample to be tested. The α-synuclein sensing film comprises a base plate and plural α-synuclein sensing polymers polymerized on the base plate, and each of the plural α-synuclein sensing polymers has plural α-synuclein detection holes thereon. The present invention also discloses a method for manufacturing an α-synuclein sensing film which comprises the steps of: mixing an α-synuclein peptide with a monomer material for a high molecular weight polymer to obtain a mixture, and polymerizing the mixture on a base plate by electropolymerization to form plural α-synuclein sensing polymer thereon. An amino acid sequence of the α-synuclein peptide of the present invention is selected from a group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3 and SEQ ID NO:4. The present invention also discloses a method for detecting α-synuclein which comprises applying a sample to be tested to an α-synuclein sensing film to detect α-synuclein in the sample. The α-synuclein sensing film comprises a base plate and plural α-synuclein sensing polymers polymerized on the base plate, and each of the plural α-synuclein sensing polymers has plural α-synuclein detection holes on a surface thereof. In an embodiment of the present invention, the base plate is a conductive base plate and the α-synuclein sensing polymers are high molecular weight polymers imprinted by an α-synuclein peptide. In an embodiment of the present invention, the conductive base plate is at least one of an indium tin oxide (ITO) base plate, a polyethylene terephthalate (PET) flexible conductive glass plate, an aluminum-doped zinc oxide (AZO) conductive base plate, a fluorine doped tin oxide (FTO) conductive base plate and a silicon dioxide conductive base plate, the high molecular weight polymer is polymerized by a monomer material comprising 3,4-ethylenefioxythiophene (EDOT), hydroxymethyl 3,4-ethylenedioxythiophene (EDOT-OH) or a combination thereof. In an embodiment of the present invention, an amino acid sequence of the α-synuclein peptide is selected from a group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3 and SEQ ID NO:4 In an embodiment of the present invention, a current density of the α-synuclein sensing film ranges from 0.01 to 500 mA. In an embodiment of the present invention the sample is selected from a group consisting of a blood sample, an urine sample, a saliva sample, a sweat sample, a tear sample, a cerebrospinal sample or a sample collected from a tissue or an organ of an organism. Accordingly, the α-synuclein sensing film of the present invention is manufactured by lectropolymerization a mixture containing α-synuclein peptide and a monomer material for a high molecular weight polymer on a base plate to obtain an α-synuclein sensing polymer. The α-synuclein sensing polymer specifically recognizes and binds the α-synuclein, and is convenient to use.

備註

連絡單位 (專責單位/部門名稱)

研究發展處

連絡電話

07-5919100