DETECTION METHOD FOR QUATERNARY AMMONIUM COMPOUND HAVING γ-CARBOXYL GROUP

專利類型

發明

專利國別 (專利申請國家)

美國

專利申請案號

15/616,734

專利證號

US 10,703,969

專利獲證名稱

DETECTION METHOD FOR QUATERNARY AMMONIUM COMPOUND HAVING γ-CARBOXYL GROUP

專利所屬機關 (申請機關)

高雄醫學大學

獲證日期

2020/07/07

技術說明

肉鹼及其相關化合物具有診斷疾病、作為治療藥物、作為食品添加、作為化妝品活性成分等實質功能，但其結構不易使用螢光偵測器偵測且其水溶性極高，不容易從水樣品中萃取出來。因此我們利用4-bromomethyl biphenyl這個試劑與肉鹼及其相關化合物進行化學衍生反應，提高螢光偵測器的偵測能力，並利用水性溶劑分散液液微萃取法(aqueous solvent-based dispersive liquid-liquid microextraction)提高水溶性小分子的萃取能力。 此方法中探討了影響衍生及萃取效率之參數，定量線性範圍為10-2000 nM，偵測極限於3 nM。同日內與異日間分析精密度(相對標準偏差)與精確度(相對誤差)小於5.5 %。我們成功地將此萃取及分析法應用於藥品、化粧品、食品、人體血漿中分析肉鹼、丁基甜菜鹼及乙醯肉鹼等水溶性待測物。 L-carnitine and its relative compounds can be used in many field such as fatty acid disorder diagnosis, pharmaceuticals, food additives, and active ingredients in cosmetics. But it’s difficult to determinate L-carnitine and its relative compounds by fluorescence detection and is also challenge to extract form water samples owing to its structures. According to their physical and chemical properties, 4-bromomethyl biphenyl was utilized as a chemical derivatization reagent to improve the detection sensitivity. The aqueous solvent-based dispersive liquid-liquid microextraction was employed to enhance the extractability in various samples. Several parameters of these derivatization and extraction efficiency were explored. The linear range of these analytes was 10-2000 nM and the limitation of detection were about 3 nM. This method is successfully applied in pharmaceuticals, cosmetics, food and human plasma for butyrobetaine, L-carnitine and acetyl-L-carnitine analysis.

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