TANDEMLY REPEATED ANTIBODY-BINDING PROTEIN AND ITS APPLICATIONS

專利類型

發明

專利國別 (專利申請國家)

美國

專利申請案號

15/649,184

專利證號

US 11,320,427 B2

專利獲證名稱

TANDEMLY REPEATED ANTIBODY-BINDING PROTEIN AND ITS APPLICATIONS

專利所屬機關 (申請機關)

臺北醫學大學

獲證日期

2022/05/03

技術說明

本發明利用基因工程技術，將可專一性結合抗體Fc (fragment-crystllizable)區域之protein G建構成串聯式重複蛋白質(poly-protein G)。Poly-protein G抓取抗體之效率與數量遠優於傳統單一protein G。Poly-protein G可固定於固相平台(solid phase plate)，提升固相平台表面之抗體乘載量，並使抗體呈現之方向性一致；poly-protein G之另一應用，可直接與抗體混合，形成poly-protein G/抗體複合物，大幅提升抗體聚集於抗原區域之數量。本發明之另一應用係將poly-protein G表現於細胞表面，形成poly-protein G細胞；將poly-protein G細胞固定於固相平台，提升固相平台表面之抗體乘載量，並使抗體呈現之方向性一致；poly-protein G細胞之另一應用，可直接與抗體混合，形成poly-protein G細胞/抗體複合物，大幅提升抗體聚集於抗原區域之數量。與poly-protein G或poly-protein G細胞搭配之抗體可不須純化，即可使用於免疫測定法。本發明能大幅提升各種抗體相關免疫測定法之偵測敏感度與極限值。 The present invention relates to a recombinant protein, named poly-protein G, which is constructed from the fragment crystallisable (Fc)-binding domain of protein G by gene engineering technologies. Poly-protein G exhibits a much greater binding efficiency and affinity to antibody than monomer protein G does. By coating poly-protein G on a solid phase plate (e.g. plate, membrane and bead), the antibody-loading amount on the plate can significantly increase and the display of antigen binding domain (Fab) of antibody on the plate can be unidirectional (outward). Another application of poly-protein G is to generate poly-protein G/antibody complexes by simply mixing poly-protein G with antibodies, and increases the accumulated amount of the antibodies on the antigen area. Moreover, the invention relates to a poly-protein G cell, which expresses poly-protein G on cell surface. By coating poly-protein G cells on a solid phase plate, the antibody-loading amount on the plate can significantly increase and the display of antigen binding domain (Fab) of antibody on the plate can be unidirectional (outward). Another application of poly-protein G cells is to generate poly-protein G cell/antibody complexes by simply mixing the cells with antibodies, and increases the accumulated amount of the antibodies on the antigen area. Pairing with poly-protein G or poly-protein G cells allows antibodies directly appling to immunoassays without additional puritification. The invention can dramatically improve the sensitivities and detection limits of immunoassays using various antibodies.

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